蜂糖李种质鉴定中孢粉学与InDel标记的应用  

Application of palynology and InDel markers in germplasm identification in Fengtangli plum

作　　者：解为玮 徐丹彤 陈方策 宋贞富 钟思玲 敖艳飞 安星 王红林 何业华[1] 郑乾明 刘朝阳 XIE Weiwei;XU Dantong;CHEN Fangce;SONG Zhenfu;ZHONG Siling;AO Yanfei;AN Xing;WANG Honglin;HE Yehua;ZHENG Qianming;LIU Chaoyang(State Key Laboratory of Subtropical Agricultural Biological Resource Conservation and Utilization/College of Horticulture,South China Agricultural University,Guangzhou 510642,Guangdong,China;Anshun Academy of Agricultural Sciences,Anshun 561000,Guizhou,China;Institute of Pomology Science,Guizhou Academy of Agricultural Science/Ministry of Agriculture and Rural Affairs Key Laboratory of Crop Genetic Resources and Germplasm Innovation in Karst Region,Guiyang 550006,Guizhou,China)

机构地区：[1]亚热带农业生物资源保护与利用国家重点实验室·华南农业大学园艺学院,广州510642 [2]安顺市农业科学院,贵州安顺561000 [3]贵州省农业科学院果树科学研究所·农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵阳550006

出　　处：《果树学报》2024年第7期1297-1309,共13页Journal of Fruit Science

基　　金：广州市科技计划项目(202206010038);安顺市科技计划项目(安市科农重[2020]01号);贵州省科技计划项目(黔科合支撑[2022]重点018号)。

摘　　要：【目的】寻找能够特异性地区分蜂糖李种质和其他李种质,以及蜂糖李中的不同品系(黄皮、青皮和一点红)的鉴定方法。【方法】选择蜂糖李中的3个品系(黄皮、青皮和一点红)及其他9个李种质作为研究对象,利用扫描电子显微镜(SEM)观察花粉形态并进行聚类分析,同时结合重测序数据进行分子标记开发,并利用21份其他李种质对分子标记的准确性进行验证。【结果】扫描电镜结果表明,蜂糖李与四月李、三月李在花粉形态上存在显著差异,而一点红蜂糖李与其他蜂糖李相比在花粉外壁纹饰上也存在一定差异。在分子标记开发方面,筛选出2对表现稳定的InDel分子标记,联合使用能够将蜂糖李与其他21份李种质有效区分,同时还筛选出一对InDel分子标记,可用于区分蜂糖李中的3个品系。【结论】研究结果为蜂糖李的种质鉴定提供了重要的孢粉学和分子标记依据,有望对蜂糖李产业的标准化与品质提升提供帮助。【Objective】Fengtangli,a superior Chinese plum variety that was bred from the local landraces in Zhenning county of Anshun City,is renowned for its crisp and tender texture.In recent years,the increasing market demand for Fengtangli seedlings has resulted in a proliferation of inferior and counterfeit seedlings being marketed as high-quality products.On the market,the visually similar Siyueli has been seen falsely sold as Fengtangli,consequently disrupting the market equilibrium.Additionally,distinct lines within the Fengtangli variety have been identified,exhibiting many variations such as yellow-skinned,green-skinned,and partially red-skinned fruits upon maturation.These phenotypic variations not only affect the fruit quality but also the required cultivation and management practices.The presence of mixed lines complicates standardized orchard management,harvest scheduling and fruit grading processes,negatively impacting the overall economic yield of the orchards.Hence,it is imperative to accurately identify and differentiate the intrinsic variations within Fengtangli as well as between Fengtangli and other plum germplasms to advance standardization and augment the quality control in the Fengtangli industry.【Methods】Flower buds were collected from 5-year-old fruit trees in the orchard at the stage of flower bud swelling to white bell,and pollen was collected after air-drying under natural conditions.Gold-spray treatment was performed using an ion sputtering instrument under vacuum,followed by observation of pollen micro-morphology under a scanning electron microscope.Young leaves from the plum germplasms were sampled for DNA extraction via an optimized CTAB method.Targeted InDel loci were identified from resequencing data using bioinformatics tools,and primers for these loci were designed.Amplification via PCR and validation of the InDel markers were performed,with the confirmation conducted through the agarose gel electrophoresis.【Results】This study conducted a systematic analysis of the pollen micr

关 键 词：蜂糖李 孢粉学 INDEL 分子标记 种质鉴定 

分 类 号：S662.3[农业科学—果树学]