高效液相色谱法同时测定果蔬中有机酸和维生素C含量  

Simultaneous determination of organic acids and VC content in fruits and vegetables using high performance liquid chromatography

作　　者：李红艳[1] 李丽梅[1] 钱训[1] 张嘉坤 郑振山[1] 陈勇达[1] LI Hong-yan;LI Li-mei;QIAN Xun;ZHANG Jia-kun;ZHENG Zhen-shan;CHEN Yong-da(Institute of Biotechnology and Food Science,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China)

机构地区：[1]河北省农林科学院生物技术与食品科学研究所,石家庄050051

出　　处：《湖北农业科学》2024年第7期171-175,184,共6页Hubei Agricultural Sciences

基　　金：河北省重点研发计划项目(21326803D);河北省农林科学院科技创新专项课题(2022KJCXZX-SSS-7)。

摘　　要：采用1%草酸(含10%甲醇)溶液提取果蔬的有机酸(酒石酸、柠檬酸、苹果酸、丁二酸、反丁烯二酸)和维生素C,色谱柱为InertSustain C18(250 mm×4.6 mm,5μm),流动相A为0.1%磷酸水溶液,流动相B为甲醇,梯度洗脱,进样体积为20μL,柱温为30℃,流速为0.8 mL/min,在210 nm和254 nm波长处分别检测5种有机酸和维生素C的含量。结果表明,该方法在标准曲线范围内线性关系良好,相关系数(R^(2))均大于0.9990,检出限为0.01~0.90μg/mL,定量限为0.15~5.00μg/mL,方法的相对标准偏差为2.40%~3.75%,回收率在86.4%~115.2%;该方法具有分析速度快、灵敏度高、重复性好、效率高等优点,可用于果蔬中有机酸和维生素C的含量测定。Using a 1%oxalic acid(containing 10%methanol)solution to extract organic acids(tartaric acid,citric acid,malic acid,succinic acid,fumaric acid)and VC from fruits and vegetables,the chromatographic column was InertSustain C18(250 mm×4.6 mm,5μm),with 0.1%phosphoric acid aqueous solution as mobile phase A and methanol as mobile phase B,gradient elution,injection volume of 20μL,column temperature of 30℃,and flow rate of 0.8 mL/min.The content of 5 organic acids and was detected VC at wavelengths of 210 nm and 254 nm,respectively.The results showed that the method had a good linear relationship within the stan-dard curve range,with correlation coefficients(R^(2))all greater than 0.9990,detection limits of 0.01~0.90μg/mL,quantification lim-its of 0.15~5.00μg/mL,relative standard deviation of 2.40%~3.75%,and recovery rates of 86.4%~115.2%;this method had the ad-vantages of fast analysis speed,high sensitivity,good repeatability,and high efficiency,and could be used for the determination of or-ganic acids and VC content in fruits and vegetables.

关 键 词：高效液相色谱法 果蔬 有机酸 维生素C 含量 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]