肝豆补肾汤对雌性Wilson病模型TX小鼠下丘脑-垂体生殖内分泌的作用研究  

Study on the Effect of Gandou Bushen Decoction on the Hypothala mus-pituitary Reproductive Endocrine in Female Wilson Disease Model TX Mice

作　　者：王路瑶 陈秋莹 韩辉[1] 房新如 赵丹 刘千琢 吴丽敏[2] WANG Luyao;CHEN Qiuying;HAN Hui;FANG Xinru;ZHAO Dan;LIU Qianzhuo;WU Limin(The First Affiliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230031,China;The First Affiliated Hospital of University of Science and Technology of China,Anhui Hefei 230031,China)

机构地区：[1]安徽中医药大学第一附属医院,安徽合肥230031 [2]中国科学技术大学附属第一医院,安徽合肥230001

出　　处：《中医药临床杂志》2024年第7期1321-1328,共8页Clinical Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(NO.82374212,NO.81673811,NO.81971446);安徽省科技厅临床转化项目(NO202204295107020044);安徽省教育厅重点项目(NO.2022AH050523)。

摘　　要：目的:探讨肝豆补肾汤对雌性Wilson病TX小鼠下丘脑-垂体生殖内分泌的作用。方法:TX和正常对照小鼠随机分为对照组、Wilson病模型组、肝豆补肾汤,每组10只。观察雌性Wilson病TX小鼠下丘脑、垂体组织病理形态及超微结构是否发生变化。ELISA法检测小鼠血清中GnRH、FSH、LH、E_(2)含量,苏木精-伊红染色法观察小鼠下丘脑和垂体组织病理形态结构变化,小鼠促性腺激素细胞超微结构通过透射电镜观察,GnRH蛋白在小鼠下丘脑组织中的表达通过免疫组化法检测,并测定其平均光密度水平。观察小鼠妊娠率及产仔数。结果:与正常对照组相比,模型组TX小鼠血清中的GnRH、FSH、LH、E_(2)水平均下降(P<0.05),下丘脑、垂体内神经元数目减少,大量神经元出现核固缩,细胞边界模糊,具有严重的病理学变化,妊娠率及产仔率下降;与模型组相比,肝豆补肾汤组小鼠血清中的GnRH、FSH、LH、E_(2)水平均上升(P<0.05),下丘脑神经元数量相较于模型组增多,垂体组织细胞组织水肿及空泡样改变减少,GnRH蛋白表达升高(P<0.05),妊娠率及产仔率上升。结论:肝豆补肾汤可通过下丘脑-垂体对生殖功能的调控作用,改善生殖激素分泌水平,上调GnRH蛋白的表达水平,改善雌性Wilson病TX小鼠生殖损害。Objective:To investigate the effect of Gandou Bushen Decoction on the reproductive endocrine of hypothalamus-pituitary in female Wilson’s disease TX mice.Methods:TX mice and normal control mice were randomly divided into control group,Wilson’s disease model group and Gandou Bushen Decoction group,10 mice in each group.The pathological morphology and ultra-structure of hypothalamus and pituitary tissues in female Wilson’s disease TX mice were observed.The levels of GnRH,FSH,LH and E_(2) in serum of mice were detected by ELISA method,the pathological morphology and structural changes of hypothalamus and pituitary tissues of mice were observed by hematoxylin-eosin staining method,the ultra-structure of mouse gonadotropin cells was observed by transmission electron microscopy,the expression of GnRH protein in mouse hypothalamus tissue was detected by immunohistochemistry,and its average optical density level was determined.The pregnancy rate and litter size of mice were observed.Results:Compared with the normal control group,the levels of GnRH,FSH,LH,and E_(2) in the serum of the model group TX mice were decreased(P<0.05),the number of neurons in the hypothalamus and pituitary decreased,a large number of neurons showed nuclear condensation,blurred cell boundaries,severe pathological changes,and decreased pregnancy rate and litter birth rate;compared with the model group,the levels of GnRH,FSH,LH,and E_(2) in the serum of the mice in the Gandou Bushen Decoction group were increased(P<0.05),the number of hypothalamic neurons increased compared with the model group,the edema and vacuolar changes of pituitary tissue cells decreased,the expression of GnRH protein increased(P<0.05),and the pregnancy rate and litter birth rate increased.Conclusion:Gandou Bushen Decoction can improve the secretion level of reproductive hormones and up-regulate the expression level of GnRH protein through the regulation of hypothalamus-pituitary on reproductive function,thereby improving the reproductive damage of female Wilson’s dise

关 键 词：肝豆补肾汤 TX小鼠 下丘脑 垂体 生殖系统损害 

分 类 号：R742.4[医药卫生—神经病学与精神病学]