妊娠期龈炎前列腺素E2的表达及清热安胎膏干预作用初探  

Expression of Prostaglandin E2 in Gingivitis During Pregnancy and the Intervention Effect of Qingr e Antai Ointment

作　　者：王楚睿 陈世娟[1] 阳亚男 陆静宇 WANG Churui;CHEN Shijuan;YANG Yanan;LU Jingyu(The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Hunan Changsha 410006,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410006

出　　处：《中医药临床杂志》2024年第7期1340-1344,共5页Clinical Journal of Traditional Chinese Medicine

基　　金：湖南省自然科学基金项目(NO.211142196052)。

摘　　要：目的:构建SD大鼠妊娠期龈炎模型,观察清热安胎膏对妊娠期龈炎前列腺素E2(PGE2)表达的影响。方法:18只SPF级SD雌鼠随机分为空白组、模型组、中药组,每组各6只,分别记录3组大鼠体重,空白组:在10%水合氯醛腹腔注射麻醉下,但不做处理,常规受孕;对照组:在10%水合氯醛腹腔注射麻醉下使用结扎法+10%蔗糖水制备大鼠实验性牙龈炎。中药组:在10%水合氯醛腹腔注射麻醉下使用结扎法+10%蔗糖水制备大鼠实验性牙龈炎,受孕成功后应用清热安胎膏填塞龈沟。三组大鼠受孕后将孕鼠单独分笼,并使三组大鼠自然妊娠至分娩子代大鼠,记录每只新生仔鼠的数量及体重,使用ELISA法检测各阶段三组大鼠龈沟液PGE2水平,记录牙龈指数(gingival index,GI)及新生仔鼠体重、数目及窝重,比较清热安胎膏的治疗效果。结果:中药组使用清热安胎膏治疗后,与模型组比较,临床GI有所降低,龈沟液PGE2表达下调(P<0.05)。结论:使用清热安胎膏对妊娠期龈炎大鼠进行治疗,大鼠牙龈红肿状况明显缓解,GI评分降低,龈沟液PGE2表达下调,治疗效果显著。Objective:To establish a SD rat model of pregnancy gingivitis and observe the effect of Qingre Antai paste on the expression of prostaglandin E2(PGE2)in pregnancy gingivitis.Methods:According to the randomization principle,18 SPF SD female rats were divided into a blank group,a model group,and a Chinese medicine group,with 6 rats in each group.The body weights of the three groups of rats were recorded.The blank group:The rats were conceived routinely under anesthesia with 10%chloral hydrate intraperitoneal injection,but no treatment was given;the control group:The rats were induced to have experimental gingivitis by using the ligation method+10%sucrose water under anesthesia with 10%chloral hydrate intraperitoneal injection.The Chinese medicine group:The rats were induced to have experimental gingivitis by using the ligation method+10%sucrose water under anesthesia with 10%chloral hydrate intraperitoneal injection,and the gingival sulcus was filled with Qingre Antai paste after successful conception.After conception,the three groups of rats were separated into separate cages,and the three groups of rats were allowed to conceive naturally until the birth of offspring rats.The number and weight of each newborn rat were recorded.The ELISA method was used to detect the PGE2 level in the gingival crevicular fluid of the three groups of rats at each stage.The gingival index(GI)and the weight,number and litter weight of the newborn rats were recorded,and the therapeutic effect of Qingre Antai Paste was compared.Results:After the Chinese medicine group was treated with Qingre Antai Paste,the clinical GI was reduced compared with the model group,and the expression of PGE2 in the gingival crevicular fluid was downregulated(P<0.05).Conclusion:Qingre Antai Paste was used to treat rats with gingivitis during pregnancy,and the redness and swelling of the gums of the rats were significantly relieved,the GI score was reduced,and the expression of PGE2 in the gingival crevicular fluid was downregulated,and the therapeutic effect

关 键 词：妊娠期龈炎 清热安胎膏 前列腺素E2 早产低体重出生儿 不良妊娠结局 

分 类 号：R781.41[医药卫生—口腔医学]