餐厨易腐有机垃圾中油脂降解菌的筛选和产酶条件优化  

SCREENING OF LIPID-DEGRADING BACTERIA AND OPTIMIZATION OF ENZYME-PRODUCING CONDITION FOR PERISHABLE KITCHEN ORGANIC WASTE

作　　者：龙腾发[1,2] 林清钰 张忠兰 孙雨暄 金尧 霍强 陈春强 LONG Tengfa;LIN Qingyu;ZHANG Zhonglan;SUN Yuxuan;JIN Yao;HUO Qiang;CHEN Chunqiang(College of Environment and Resources,Guangxi Normal University,Guilin 541004,China;Key Laboratory of Ecology and Environmental Protection of Rare and Endangered Plants and Animals,Guilin 541004,China)

机构地区：[1]广西师范大学环境与资源学院,广西桂林541004 [2]珍稀濒危动植物生态与环境保护省部共建重点实验室,广西桂林541004

出　　处：《环境工程》2024年第6期103-110,共8页Environmental Engineering

基　　金：国家自然科学基金项目(51968007)。

摘　　要：近年来,我国餐厨易腐有机垃圾产生量逐年上升,为提高其降解速率,亟须开发高效降解餐厨易腐有机垃圾的工艺。筛选了能够适应餐厨易腐有机垃圾且能高效降解油脂能力的菌株,以期实现餐厨易腐有机垃圾快速降解和资源化。从餐厨易腐有机垃圾及部分被油污染的土壤中采集样品,采用16S rDNA测序技术并构建菌株发育树,利用响应面分析方法研究菌株最适生长条件。通过筛选及功能验证,获得1株高效降解油脂菌株TH1,初步鉴定为粘质沙布雷氏菌(Serratia marcescens)。单因素实验和响应面方法表明,该菌株在温度30℃、pH=7.54、盐度1%的条件下,脂肪酶活力高达213.70 U/L。该研究可为后续开发复合菌剂进行餐厨易腐有机垃圾高效降解、机理研究及资源化利用提供种质资源。In recent years,the production of perishable organic waste in kitchens has been increasing.To improve the degradation rate,it is urgent to develop an efficient process for degrading perishable organic waste in the kitchen.In this paper,we screened a bacterial strain that can adapt to perishable organic waste in the kitchen and degrade oil efficiently.Samples were collected from perishable organic waste and oil-contaminated soil,and 16S rDNA sequencing technology was used to construct a strain growth tree,and response surface analysis was used to study the optimal growth conditions of the strain.Through screening and functional verification,a lipid-degrading strain,named as TH1,was obtained and identified as Serratia marcescens.The single-factor experiment and response surface method showed that the lipase activity reached 213.70 U/L under the condition of temperature of 30℃,pH=7.54,and salinity of 1%.The results can provide germplasm resources for the subsequent development of composite bacterioides for efficient degradation,mechanism research,and resource utilization of perishable kitchen organic waste.

关 键 词：油脂降解 餐厨易腐有机垃圾 酶活力 响应面优化 

分 类 号：X799.3[环境科学与工程—环境工程] X172