长顺绿壳蛋鸡GRIN1基因3′非翻译区的多态性及其与蛋壳品质的关联性  

Polymorphism of 3′ Untranslated Region in GRIN1 Gene and Its Correlation with Eggshell Quality in Changshun Green-shell Layer

作　　者：罗华伦 王春源 吴燕 向进 张依裕 LUO Hualun;WANG Chunyuan;WU Yan;XIANG Jin;ZHANG Yiyu(Qianxinan Prefecture Agricultural Products Quality and Safety Inspection Center,Xingyi 562400,China;College of Animal Science,Guizhou University,Key Laboratory of Genetic Breeding and Reproduction of Plateau Mountainous Animals,Ministry of Education,Guiyang 550000,China)

机构地区：[1]黔西南州农产品质量安全检测中心,贵州兴义562400 [2]贵州大学动物科学学院,高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550000

出　　处：《云南农业大学学报（自然科学版）》2024年第3期56-63,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：贵州省科技合作计划项目[黔科合LH字(2016)7454号]。

摘　　要：【目的】探索长顺绿壳蛋鸡GRIN1基因3′非翻译区(untranslated region,UTR)多态性对蛋壳品质的影响。【方法】选择185只健康蛋鸡,测定其第45周龄产蛋的蛋质量、蛋形指数、蛋壳强度、蛋壳厚度和蛋壳质量5个指标;使用NCBI和PrimerPremier 3.0设计引物,采用正向测序法筛选GRIN1基因在3′UTR区域的SNP位点。【结果】GRIN1基因的3′UTR检测到4个SNP位点:g.30871C>T、g.31017A>G、g.31158A>C和g.31166G>C,其中仅g.31158A>C和g.31166G>C之间存在强连锁不平衡,且g.30871C>T和g.31017A>G都极显著偏离哈代—温伯格平衡(P<0.01)。g.31017A>G与蛋壳品质的关联分析中,GG基因型的蛋质量显著高于AA基因型(P<0.05)。4个SNP位点共产生5个单倍型(H1、H2、H3、H4、H5)和8个双倍型(H1H1、H1H2、H1H3、H2H2、H2H3、H2H4、H3H5、H4H4),双倍型H3H5的蛋质量显著高于双倍型H2H2和H2H3(P<0.05),双倍型H3H5的蛋壳质量显著高于双倍型H2H2(P<0.05),其他双倍型指标间的差异未达到显著水平(P>0.05)。【结论】长顺绿壳蛋鸡GRIN1基因与蛋壳品质存在显著关联;g.31017A>G对蛋壳品质有显著影响,能够作为改善蛋壳品质的分子标记位点参考。[Purpose]To explore the effects of polymorphism of 3′ untranslated region(UTR) in GRIN1 gene on eggshell quality in Changshun green-shell layer.[Methods]A total of 185 healthy layers were selected for egg production at 45 weeks of age.Egg weight,egg shape index,eggshell strength,eggshell thickness and eggshell weight were measured.Primers were designed by NCBI and PrimerPremier 3.0.SNPs in the 3′ UTR region of GRIN1 gene were screened by forward sequencing.[Results]Four SNPs,including g.30871C>T,g.31017A>G,g.31158A>C and g.31166G>C,were detected in the 3′ UTR region of GRIN1 gene,but only g.31158A>C and g.31166G>C showed strong linkage disequilibrium,and g.30871C>T and g.31017A>G deviated significantly from Hardy-Weinberg equilibrium.In the association analysis between g.31017A>G and eggshell quality,the egg weight of genotype GG was significantly higher than that of genotype AA(P<0.05).Five haplotypes(H1,H2,H3,H4,H5) and eight diplotypes(H1H1,H1H2,H1H3,H2H2,H2H3,H2H4,H3H5,H4H4) were produced by four SNP loci.The egg weight of diplotype H3H5 was significantly higher than that of diplotype H2H2 and H2H3(P<0.05),and the eggshell weight of diplotype H3H5 was significantly higher than that of diplotype H2H2(P<0.05),but there were no significant differences among indexes of other diplotypes(P>0.05).[Conclusion]GRIN1 gene in Changshun green-shell layer is significantly correlated with eggshell quality,and g.31017A>G has significant effect on eggshell quality,which can be used as a reference for improving eggshell quality.

关 键 词：长顺绿壳蛋鸡 GRIN1基因 3′非翻译区 SNP位点 蛋壳品质 

分 类 号：S831.2[农业科学—畜牧学]