铁皮石斛接种菌根真菌的生理效应研究  

Physiological Effects of Inoculation of Mycorrhizal Fungi on Dendrobium officinale

作　　者：余宇潇 郑焙华 王河川 李花 刘建福[1] 吴新泉 Yu Yuxiao;Zheng Beihua;Wang Hechuan;Li Hua;Liu Jianfu;Wu Xinquan(Institute of Horticultural Science and Engineering,Huaqiao University,Xiamen,Fujan 361021,China;Quanzhou Forestry Bureau,Quanzhou,Fujian 362000,China;Quanzhou Crop Production Technology Station,Quanzhou,Fujian 362000,China;Quanzhou Quanfeng Biotechnology Co.,Ltd.,Quanzhou,Fujian 362021,China)

机构地区：[1]华侨大学园艺科学与工程研究所,福建厦门361021 [2]泉州市林业局,福建泉州362000 [3]泉州市种植业技术站,福建泉州362000 [4]泉州市泉丰生物科技有限公司,福建泉州362021

出　　处：《东南园艺》2024年第2期117-124,共8页Southeast Horticulture

基　　金：福建省高校产学合作项目(2022N5016)。

摘　　要：【目的】探究不同菌根真菌与铁皮石斛共生的菌根效应,揭示共生生理效应,为石斛菌根化栽培提供理论依据。【方法】以铁皮石斛组培苗为研究对象,在其根部接种耐盐枝孢霉菌(Cladosporium halotolerans,Ch)和石斛小菇(Mycena dendrobii,Md)2种菌根真菌,分析菌根真菌对铁皮石斛生长发育、生物活性物质以及糖转运情况的影响。【结果】在铁皮石斛组培苗接种菌根真菌10 d后,根部表面出现褐化现象,在根部皮层细胞内形成具有螺旋状或念珠状结构的菌丝结。接种Md和Ch后铁皮石斛生物量变化均不显著。接种Md后地上部和地下部生物碱含量显著提高,分别比未接种处理提高21.74%和18.75%,地上部总黄酮含量提高30.00%;接种Md后地上部果糖含量显著提高25.00%,并促进地上部葡萄糖和蔗糖转运到地下部。铁皮石斛接种Ch地上部生物碱含量显著提高12.77%,而地下部生物碱含量显著降低43.75%;地上部和地下部总黄酮含量分别降低27.12%和50.91%;接种Ch地上部果糖含量显著提高30.00%,促进地下部蔗糖转运到地上部。【结论】铁皮石斛接种菌根真菌10d已形成共生关系,菌根真菌调节铁皮石斛生物碱和总黄酮等生物活性物质的生成,促进果糖、葡萄糖和蔗糖的转运。【Objective】To investigate the mycorrhizal effects of different fungi in symbiosis with Dendrobium officinale,reveal the physiological effects of orchid mycorrhizal symbiosis,and provide theoretical basis for mycorrhizal cultivation of Dendrobium officinale.【Method】Using tissue culture seedlings of Dendrobium officinale as the research object,two mycorrhizal fungi,Cladosporium halotolerans(Ch)and Mycena dendrobii(Md),were inoculated to analyze their effects on the growth and development,bioactive substances,and sugar transport of Dendrobium officinale.【Result】After 10 days of inoculation with two types of fungi in the tissue culture seedlings of Dendrobium officinale,mycelial nodules with spiral and moniliform structures were formed in the cortical cells of the roots,and browning appeared on the surface of the Dendrobium officinale roots.Following inoculation with Md and Ch,no significant change was observed in the biomass of Dendrobium officinale;Following inoculation with Md,the alkaloid content in both the aboveground and underground parts rose by 21.74%and 18.75%,respectively,with the total flavonoid content in the aboveground parts increasing by 30%;Inoculation with Md led to a 25.00%increase in fructose content in the aboveground parts and facilitated the transport of glucose and sucrose from the aboveground to the underground parts.Inoculation with Ch led to a 12.77%increase in alkaloid content in the aboveground parts of Dendrobium officinale,but a 43.75%decrease in the underground parts;The total flavonoid content decreased by 27.12%in the aboveground parts and 50.91%in the underground parts;Inoculation with Ch resulted in a 30.00%increase in fructose content in the aboveground parts and facilitated the transport of sucrose from the underground to the aboveground parts.【Conclusion】After being inoculated with mycorrhizal fungi,Dendrobium officinale forms a symbiotic relationship.The mycorrhizal fungi enhance the production of alkaloids and flavonoids in Dendrobium officinale,and facilitate

关 键 词：铁皮石斛 菌根效应 生长发育 生物活性物质 糖转运 

分 类 号：S567.239[农业科学—中草药栽培]