An Efficient Probe for Bacterial Nitroreductase Imaging and Detection Based on NanoLuc-Furimazine Bioluminescent Pair  

作　　者：Ximeng Shi Yumeng Wang Jiaoyang Yu Yating Yang Zecheng Jin Hanghang Wang Dalei Wu Wei Chen Jianming Guo Yinan Zhang 

机构地区：[1]Jiangsu Key LaboratoryforFunctional Substances of Chinese Medicine,School of Pharmacy,Nanjing Universityof Chinese Medicine,Nanjing,Jiangsu210023,China [2]Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,School of Pharmacy,Nanjing University of ChineseMedicine,Nanjing,Jiangsu210023,China [3]Clinical Research Center,the Second Hospital of Nanjing,Affiliated Hospital to Nanjing University of Chinese Medicine,Nanjing,Jiangsu210003,China [4]Key Laboratory of Resources Biology and Biotechnology in Western China,Ministry of Education,College of Life Sciences,NorthwestUniversity,Xi'an,Shaanxi710069,China [5]Helmholtz International Lab,State Key Laboratoryof Microbial Technology,Shandong University,Qingdao,Shandong 266237,China

出　　处：《Chinese Journal of Chemistry》2024年第12期1373-1380,共8页中国化学（英文版）

基　　金：supported by the Program of the National Natural Science Foundation of China(No.22177052,Y.Zhang,No.82074136,J.Guo,No.82272340,W.Chen);the Joint Training Program for Graduated Students of the Institute of Translational Research and Drug Innovation(X.Shi).

摘　　要：Comprehensive SummaryThe detection of critical endogenous species, such as bacteria in microenvironments in the body, requires better imaging tools for visualization and monitoring of biological events. Bioluminescence imaging is the most popular strategy for obtaining real-time in living cells and organisms. Herein, we introduced a nitroaryl group on the C-3 position and a hydroxy group at the C-6 phenyl ring on furimazine to report the first bioluminescent probe (7) based on NanoLuc-furimazine bioluminescent pair for the detection of nitroreductase in bacteria. The probe, which possessed up to 560-fold intensity increase with a low detection limit of 16 ng/mL of nitroreductase, has the most efficient uncage efficiency in comparison with other bioluminescent congeners, thus enabling highly selective and sensitive visualization of NTR activity in a panel of clinical priority pathogens. Additionally, imaging of the recombinant strain as well as the NTR from mouse feces indicated the potential of this probe in the application of different mouse disease models.

关 键 词：NITROREDUCTASE NanoLuc-furimazine Biosensors Analytical methods LUMINESCENCE Imaging agents 

分 类 号：O657.3[理学—分析化学]