魔芋葡甘聚糖接枝聚丙烯酸敷料对糖尿病皮损治疗作用的研究  

Effects of konjac glucomannannone-polyacrylic acid dressing on diabetic skin wound healing

作　　者：陈泫锦 罗凌鹏 张闯年 孔德领[2] 李宸 CHEN Xuan-jin;LUO Ling-peng;ZHANG Chuang-nian;KONG De-ling;LI Chen(Tianjin Key Laboratory of Biomedical Materials,Institute of Biomedical Engineering,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300192,China;Key Laboratory of Bioactive Materials,Ministry of Education,College of Life Sciences,State Key Laboratory of Medici-nal Chemical Biology,Collaborative Innovation Centre of Chemical Science and Engineering,and National Institute of Functional Materials,Nankai University,Tianjin 300071,China)

机构地区：[1]中国医学科学院·北京协和医学院生物医学工程研究所天津市生物医学材料重点实验室,天津300192 [2]南开大学生命科学学院天津化学化工协同创新中心·药物化学生物学国家重点实验室·生物活性材料教育部重点实验室,天津300071

出　　处：《生物医学工程与临床》2024年第4期449-456,共8页Biomedical Engineering and Clinical Medicine

基　　金：国家重点研发计划项目(2020YFA0803704)。

摘　　要：目的探究魔芋葡甘聚糖(KGM)接枝聚丙烯酸(AA)敷料对糖尿病皮肤损伤的修复作用。方法采用自由基聚合方法制备(KGM-AA)敷料,进行吸水性实验检测KGM-AA敷料的吸水性能与黏性;通过扫描电子显微镜观察材料冻干后结构及形态。将KGM-AA水凝胶与小鼠成纤维细胞L929体外共培养24 h后,进行细胞活死染色及CCK-8检测,探究细胞的活性及生长状况。将血管内皮细胞接种到KGM、KGM-AA和空白对照上,于接种后第2、4、6、8小时时间点观察血管形成情况。通过小鼠腹腔注射STZ造糖尿病模型,当随机血糖连续2次大于11.1 mmol/L即造模成功。将所获得的高血糖糖尿病小鼠于颈后处制造直径为1 cm圆形伤口并随机分为3组,一组为不做处理空白对照组,另2组分别用KGM及KGM-AA敷料对小鼠创面进行治疗,观察伤口的愈合情况。于创伤后第7、14天时取伤口处皮肤进行苏木精-伊红(HE)染色,观察伤口愈合及炎症、增生等情况。结果KGM-AA敷料具有良好的吸水能力,吸水后的KGM-AA呈现均匀的多孔结构,孔径均大于10μm。与小鼠L929共培养后发现,细胞成功进入吸水形成的凝胶内部,细胞存活率大于95%,可以为细胞附着提供良好的环境且对细胞生长及活性无显著影响。成管实验结果显示,与KGM相比,接种6 h左右KGM-AA组血管数量明显多于KGM组,差异有显著性统计学意义(P<0.01),该差异一直维持到接种后的第10小时。体内实验表明,与对照组相比,创口用KGM-AA敷料处理过的高血糖小鼠在治疗7 d时创面皮肤比未接受治疗组更加完整。结论KGM-AA敷料吸水性及生物相容性良好,吸水后形成均匀多孔结构,形成保护膜的同时为细胞附着提供了良好生长条件,同时表现出良好的促进血管生成特性和潜在的调节皮肤损伤能力,是理想的伤口敷料和细胞递送材料。Objective To investigate the effectiveness of konjac gulcomannan(KGM)polyacrylic acid(AA)dressing on diabetic wound healing.Methods KGM-AA dressing was fabricated by free radical polymerization,and water-absorption experiments were performed to examine water-absorbing and adhesive properties of KGM-AA.The micro-structure and morphology of KGM-AA were observed using scanning electron microscopy after KGM-AA lyophilization.The mouse fibroblasts L929 cells were co-cultured with KGM-AA for 24 hours,the cell proliferation and viability were estimated using live-dead staining kit and CCK-8 assay.The vascular endothelial cells were also used for KGM-AA co-culturing,and tube formation assay was performed.The vascular tube formation was observed at 2,4,6 and 8 hours after cell seeding.The diabetic model was established by intraperitoneal injection of streptozotocin(STZ)into mice,and the random blood glucose was greater than11.1 mmol/L for two consecutive times.A circular wound with 1 cm in diameter were created on the back of mice,and the mice were randomly divided into 3 groups which were blank control group without treatment,and the other two groups which were treated with KGM and KGM-AA dressings respectively to observe wound healing effects.The hematoxylin-eosin(HE)staining was performed on wound skin after 7 and 14 days to observe wound healing,inflammation and hyperplasia.Results The KGM-AA exhibited excellent water-absorbing ability,and showed uniform porous structure with pore size of more than 10μm after water absorption.After co-cultured with mouse L929,the cells successfully embedded within the KGM-AA,and embedded cells also showed excellent viability and proliferation ability,indicated that KGM-AA provided appropriate environment for cell attachment and growth.The results of tube formation experiment showed that the number of blood vessels in KGM-AA group was significantly higher than that in KGM group at about 6 hours after seeding,and the difference was statistically significant(P<0.01).The difference was m

关 键 词：魔芋葡甘聚糖 聚丙烯酸 糖尿病 皮肤损伤 生物敷料 

分 类 号：R318.08[医药卫生—生物医学工程] R587.1[医药卫生—基础医学]