超高效液相色谱法测定番茄中番茄红素含量  

Determination of lycopene content in tomato by ultra-high performance liquid chromatography

作　　者：柯建赛 杨飞萍[1] 金叶舟 蒋武毅 KE Jiansai;YANG Feiping;JIN Yezhou;JIANG Wuyi(Wenzhou Agricultural Products Inspection and Testing Center,Wenzhou 325014,Zhejiang)

机构地区：[1]温州市农产品检验测试中心,浙江温州325014

出　　处：《浙江农业科学》2024年第7期1683-1686,共4页Journal of Zhejiang Agricultural Sciences

摘　　要：文章建立了一种利用超高效液相色谱测定番茄中番茄红素含量的方法。2.00 g样品加入10 mL丙酮,涡旋混匀提取10 min, 4℃10 000 r·min^(-1)离心5 min,取上层清液过0.22μm微孔滤膜,上机检测。结果发现,番茄红素在1.00~100.00μg·mL^(-1)浓度范围呈良好的线性关系,加标回收率为91.2%~106.7%,相对标准偏差(RSD)为2.8%~5.1%。该方法可以节省大量的时间和试剂,使前处理更简单,更经济,更环保。In this paper,a method for the determination of lycopene content in tomato by ultra-high performance liquid chromatography(UPLC)was established.10 mL of acetone was added to the 2.00 g sample,mixed with a whirl instrument for 10 min,centrifuged at 4℃at 10000 r·min^(-1) for 5 min,and the supernatant was passed through a 0.22μm microporous filter membrane for detection.The results showed that lycopene showed a good linear relationship in the concentration range of 1.00-100.00μg·mL^(-1),and the recovery rate was 91.2%-106.7%,and the relative standard deviation(RSD)was 2.8%-5.1%.This method can save a lot of time and reagents,and make the pretreatment simpler,more economical,and more environmentally friendly.

关 键 词：番茄红素 超高效液相色谱法 番茄 

分 类 号：S641.2[农业科学—蔬菜学]