NtCPS2基因通过调控赤霉素合成影响烟草腺毛发生  

NtCPS2 Gene Affects the Occurrence of Tobacco(Nicotiana tabacum)Glandular Hair by Regulating Gibberellin Synthesis

作　　者：曹可心 杨惠娟[1] 刘化冰[2] 吴健 陈小翔[2] 徐世晓[1] CAO Ke-Xin;YANG Hui-Juan;LIU Hua-Bing;WU Jian;CHEN Xiao-Xiang;XU Shi-Xiao(Tobacco College,Henan Agricultural University,Zhengzhou 450002,China;Zhejiang China Tobacco Industry Co.,Ltd.,Hangzhou 310008,China)

机构地区：[1]河南农业大学烟草学院,郑州450002 [2]浙江中烟工业有限责任公司,杭州310008

出　　处：《农业生物技术学报》2024年第8期1784-1791,共8页Journal of Agricultural Biotechnology

基　　金：中国烟草总公司河南省公司重点项目(2021410000240021);浙江中烟工业有限责任公司项目(ZJZY2021B009,2023330000340127);贵州省烟草公司铜仁市公司项目(202101)。

摘　　要：烟草(Nicotiana tabacum)叶面腺毛的生长发育与烟叶的香气品质有关。为探讨柯巴基焦磷酸合酶(Ent-copalyl diphosphate synthase 2,NtCPS2)与烟草腺毛生长发育及腺毛分泌物合成的关系,本研究使用'8306'的野生型品系(WT)、NtCPS2纯合编辑系(KD)和过表达株系(OE)为材料,对3种株系的烟草进行腺毛表型观察、腺毛密度统计、叶面分泌物西伯三烯二醇含量测定及其关键合成基因表达量进行分析。结果表明,与WT相比,KD株系的叶片分泌型腺毛密度增加75%左右,叶面腺毛分泌物显著增多(P<0.05),OE株系的叶面腺毛分泌物显著低于WT株系(P<0.05),较WT减少50%左右。负调控腺毛基因B2-型细胞周期蛋白(cyclin B2,NtCycB2)基因表达量在KD株系中显著低于WT株系(P<0.05),OE与KD株系呈现相反趋势,NtCycB2基因表达量与腺毛表型呈相反趋势。KD烟株赤霉素含量显著高于WT株系(P<0.05);OE烟株赤霉素含量显著低于WT株系(P<0.05)。KD株系赤霉素含量升高,分泌型腺毛密度增多,叶面主要腺毛分泌物西柏烷类物质含量增多;OE株系与KD株系呈相反趋势。综上结果表明,NtCPS2基因可能通过正向调控赤霉素生物合成,促进烟草分泌性腺毛密度增多,促进叶面腺毛分泌物分泌。本研究为NtCPS2基因通过赤霉素途径调控烟草腺毛及烟草香气物质的合成提供了新的研究方向。The growth and development of foliar glandular hairs in tobacco(Nicotiana tabacum)is related to the aroma quality of tobacco leaves.In order to investigate the relationship between Ent-copalyl diphosphate synthase 2(NtCPS2)and the growth and development of glandular hair and the synthesis of glandular hair secretion in tobacco,the wild-type strain(WT),NtCPS2 homozygous editing strain(KD)and overexpression strain(OE)of'8306'were used as materials to observe the phenotype of glandular hair and the density of glandular hair.Content of cyperane substances was determined and the expression of key synthetic genes was analyzed.The results showed that compared with WT,the density of leaf secretory glandular hairs in KD strains increased by about 75%,and the secretion of leaf glandular hairs increased significantly(P<0.05),while the secretion of leaf glandular hairs in OE strains was significantly lower than that of WT strains(P<0.05),and decreased by about 50%compared with WT strains(P<0.05).The expression level of cyclin B2(NtCycB2)gene in the negative regulation of glandular hair gene was significantly lower than that in WT and KD strain,and the expression level of NtCycB2 gene in OE and KD strains showed an opposite trend,and the expression level of NtCycB2 gene was opposite to that of glandular hair phenotype.The gibberellin content of KD tobacco plant was significantly higher than that of WT strain(P<0.05).The gibberellin content of OE strains was significantly lower than that of WT strains(P<0.05).The content of gibberellin in KD strains increased,the density of secretory glandular hairs increased,and the content of cyperane substances,the main glandular hair secretions in the foliar strain increased.The OE strain showed an opposite trend to the KD strain.The results suggested that the NtCPS2 gene might promote the increase of gonadal hair density and foliar glandular hair secretion by positively regulating gibberellin biosynthesis.This study provides a new research direction for the regulation of the synthesis of

关 键 词：烟草 叶面腺毛 柯巴基焦磷酸合酶(NtCPS2) 赤霉素 香气物质 代谢途径 

分 类 号：S572[农业科学—烟草工业]