Effect of miR-130a targeting phosphase and tensin homology deleted on chromosome ten/ phosphoinositol 3 kinase/protein kinase B axis on apoptosis of renal tissue cells in rats with diabetic kidney disease  

作　　者：许春梅 XU Chunmei(Dept Nephrol,Longyan 1st Hosp,Fujian Med Univ,Longyan 364000)

机构地区：[1]Dept Nephrol,Longyan 1st Hosp,Fujian Med Univ,Longyan 364000

出　　处：《China Medical Abstracts(Internal Medicine)》2024年第2期94-94,共1页中国医学文摘（内科学分册（英文版）

摘　　要：Objective To investigate the effect of miR-130a targeting phosphase and tensin homology deleted on chromosome ten(PTEN)/phosphoinositide 3 kinase(PI3K)/protein kinase B(AKT)pathway on renal tissue cell apoptosis in diabetic kidney disease(DKD)rats.Methods The DKD rat model was constructed by feeding high-sugar and high-fat diet combined with intraperitoneal injection of streptozotocin(STZ).72 rats were divided into normal control group(NC),DKD model group(DKD),miR-13Oa agonist negative control group(NC agomir),and miR-130a agonist group(miR-130a agomir),miR-130a agomir+PTEN overexpression negative control group(miR-13a agomir+pcDNA),and miR-130a agomir+PCDNA-PTEN over-expression group(miR-130a Agomir+PCDNA-PTEN),with 12 rats in each group.Urinary microalbumin kit was used to detect 24 h urine albumin(UAlb).Fasting blood glucose(FBG),serum creatinine(Scr)and blood urea nitrogen(BUN)were detected by automatic biochemical analyzer.Pathological changes of renal tissue were detected by HE staining.The levels of serum IL-6 and TNF-αwere detected by ELISA.The apoptosis of renal tissue was detected by TUNEL staining.The expression of miR-130a was detected by qRT-PCR,and the expression of B-cell lymphoma-2-associated X protein(Bax).

关 键 词：miR RATS KIDNEY 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]