高良姜素通过激活cGAS/STING信号通路抑制骨肉瘤MG63细胞的恶性生物学行为  

Galangin inhibits the malignant biological behavior of osteosarcoma MG63 cells by activating the cGAS/STING signaling pathway

作　　者：加鹏飞[1] 余小超 刘小波 李鹏程 JIA Pengfei;YU Xiaochao;LIU Xiaobo;LI Pengcheng(Department of Spine Surgery,Suizhou Central Hospital Affiliated toHubei University of Medicine,Suizhou 441300,Hubei,China;Department of Orthopedics,Huazhong University of Science and Technology,Wuhan 430022,Hubei,China;Cancer Center,Union HospitalAffiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei,China)

机构地区：[1]湖北医药学院附属随州市中心医院脊柱外科,湖北随州441300 [2]华中科技大学同济医学院附属协和医院骨科,湖北武汉430022 [3]华中科技大学同济医学院附属协和医院肿瘤中心,湖北武汉430022

出　　处：《中国肿瘤生物治疗杂志》2024年第7期675-680,共6页Chinese Journal of Cancer Biotherapy

基　　金：湖北省自然科学基金(No.2020HBA216)。

摘　　要：目的:探讨高良姜素(Gal)是否通过调节cGAS/STING信号通路影响骨肉瘤MG63细胞增殖、迁移、侵袭和凋亡。方法:体外培养人骨肉瘤MG63细胞,分别使用0、5、15、25、50、100、200μmol/L的Gal培养48 h后,CCK-8法检测Gal对细胞活力的影响。将MG63细胞分为对照组(未处理细胞)、Gal低浓度组(Gal-L组,50μmol/L Gal处理)、Gal高浓度组(Gal-H组,100μmol/L Gal处理)和Gal-H+STING抑制剂组(Gal-H+H-151组,100μmol/L Gal+8μmol/L H-151处理)。采用EdU染色法、划痕愈合实验、Transwell小室法、流式细胞术检测各组细胞增殖活力、迁移、侵袭和凋亡能力,WB法检测各组细胞中cGAS、STING蛋白的表达水平。结果:与对照组比较,Gal-L组、Gal-H组细胞增殖活力、迁移、侵袭能力均显著降低(均P<0.05),细胞凋亡率和cGAS、STING蛋白表达水平均显著升高(均P<0.05);与Gal-H组比较,Gal-H+H-151组细胞增殖活力、迁移和侵袭能力均显著升高(均P<0.05),细胞凋亡率和cGAS、STING蛋白表达水平均显著降低(均P<0.05)。结论:Gal可能通过激活cGAS/STING信号通路抑制骨肉瘤MG63细胞增殖、迁移、侵袭并促进细胞凋亡。Objective:To investigate whether galangin(Gal)affects the proliferation,migration,invasion and apoptosis of osteosarcoma MG63 cells by regulating the cGAS/STING signaling pathway.Methods:Human osteosarcoma MG63 cells were cultured in vitro and treated with Gal at concentrations of 0,5,15,25,50,100 and 200μmol/L for 48 hours,and the effect of Gal on cell viability was detected by CCK-8 method.MG63 cells were divided into the control group(untreated cells),the Gal low concentration group(Gal-L group,treated with 50μmol/L Gal),the Gal high concentration group(Gal-H group,treated with 100μmol/L Gal),and the Gal-H+STING inhibitor group(Gal-H+H-151 group,treated with 100μmol/L Gal+8μmol/L H-151).EdU staining method,scratch healing test,Transwell chamber method and flow cytometry were used to detect cell proliferation,migration,invasion and apoptosis of cells in each group.Western blot was applied to detect the expression levels of cGAS and STING proteins in cells of each group.Results:Compared with the control group,the proliferation activity,migration,and invasion abilities of the cells in the Gal-L and Gal-H groups were significantly decreased(all P<0.05);the apoptosis rate and the expression levels of cGAS and STING proteins were significantly increased(all P<0.05).Compared with the Gal-H group,the proliferation activity,migration,and invasion abilities of the cells in the Gal-H+H-151 group were significantly increased(all P<0.05);the apoptosis rate and the expression levels of cGAS and STING proteins were significantly decreased(all P<0.05).Conclusion:Gal may inhibit the proliferation,migration and invasion and promote the apoptosis of osteosarcoma cells by activating the cGAS/STING signaling pathway.

关 键 词：高良姜素 骨肉瘤 MG63细胞 增殖 迁移 侵袭 凋亡 cGAS/STING信号通路 

分 类 号：R738.1[医药卫生—肿瘤] R730.2[医药卫生—临床医学]