京尼平对下咽癌FaDu细胞增殖及线粒体功能的影响  

Effects of genipin on the proliferation and mitochondrial function of hypopharyngeal carcinoma FaDu cells

作　　者：彭瑶 周颖 高宇 刘颖[1] 徐傲枫 张畅 张春晶[1] 于海涛[4] PENG Yao;ZHOU Ying;GAO Yu;LIU Ying;XU Aofeng;ZHANG Chang;ZHANG Chunjing;YU Haitao(Departmentof Biochemistry and Molecular Biology,Qiqihar 161000,Heilongjiang,China;Blood Diagnostic Laboratory,the Second Affiliated Hospital,Qiqihar 161000,Heilongjiang,China;PhaseⅠClinical ResearchCenter,the Third Affiliated Hospital,Qiqihar 161000,Heilongjiang,China;Department of Cell Biology and Genetics,Qiqihar Medical University,Qiqihar 161000,Heilongjiang,China;Special Medical Service Department Section Four,Strategic Support Force Medical Center of PLA,Beijing100020,China)

机构地区：[1]齐齐哈尔医学院生物化学与分子生物学教研室,黑龙江齐齐哈尔161000 [2]齐齐哈尔医学院附属第二医院血液诊断室,黑龙江齐齐哈尔161000 [3]齐齐哈尔医学院附属第三医院Ⅰ期临床研究中心,黑龙江齐齐哈尔161000 [4]齐齐哈尔医学院细胞生物学与遗传学教研室,黑龙江齐齐哈尔161000 [5]中国人民解放军战略支援部队特色医学中心特勤诊疗四科,北京100020

出　　处：《中国肿瘤生物治疗杂志》2024年第7期681-686,共6页Chinese Journal of Cancer Biotherapy

基　　金：齐齐哈尔市科技局科技计划联合引导项目(No.LSFGG-2023031)。

摘　　要：目的:探讨解偶联蛋白2(UCP2)抑制剂京尼平(GEN)对人下咽癌FaDu细胞增殖及线粒体功能的影响。方法:使用不同浓度的GEN作用于FaDu细胞24 h,实验分为GEN 0(对照)、50、100、200和400μmol/L组。采用CCK-8法检测各组细胞增殖能力,DCFH-DA探针及JC-1染色联合流式细胞术检测GEN对FaDu细胞活性氧(ROS)含量及线粒体膜电位的影响,激光共聚焦显微镜观察GEN对FaDu细胞线粒体膜通透性转换孔的影响,可见分光光度法检测细胞中乳酸的含量,WB法检测细胞中UCP2蛋白的表达变化。结果:与对照组相比,GEN可显著抑制FaDu细胞的增殖活力(P<0.05或P<0.01)、细胞中UCP2蛋白的表达(P<0.05),降低线粒体膜电位(P<0.05或P<0.01)、乳酸含量(P<0.0001),改变细胞线粒体膜孔道通透性,提高细胞中ROS水平(P<0.05或P<0.01)。结论:GEN通过调节细胞中UCP2的表达水平进而影响细胞的氧化还原能力及线粒体功能,从而发挥抑制人下咽癌FaDu细胞增殖并诱导细胞凋亡的作用。Objective:To explore the effects of UCP2 inhibitor genipin(GEN)on the proliferation and mitochondrial function of human hypopharyngeal carcinoma FaDu cells.Methods:FaDu cells were treated with different concentrations of GEN for 24 hours and divided into the GEN 0μmol/L(control)group,the 50μmol/L group,the 100μmol/L group,the 200μmol/L group and the 400μmol/L group.The CCK-8 method was employed to assess cell proliferation,and the DCFH-DA probe and JC-1 flow cytometry were utilized to measure the impact of GEN on reactive oxygen species(ROS)levels and mitochondrial membrane potential in FaDu cells.Laser confocal microscopy was utilized to observe the effect of GEN on the mitochondrial membrane permeability transition pore(MPTP)in FaDu cells.Spectrophotometry was employed to measure lactate levels in cells,and Western blot analysis was conducted to monitor changes in UCP2 protein expression.Results:In comparison with the control group,GEN significantly inhibited the proliferation activity of FaDu cells(P<0.05,P<0.01),reduced the expression of UCP2 protein in cells(P<0.05),decreased the mitochondrial membrane potential(P<0.05,P<0.01)and lactate content(P<0.001),altered mitochondrial membrane permeability,and increased the levels of ROS in cells(P<0.05,P<0.01).Conclusion:GEN modulates the expression of UCP2 in cells,consequently altering their redox potential and mitochondrial function,thus inhibiting the viability and inducing apoptosis in human nasopharyngeal carcinoma FaDu cells.

关 键 词：下咽癌 京尼平 解偶联蛋白2 FaDu细胞 增殖 凋亡 线粒体损伤 

分 类 号：R739.63[医药卫生—肿瘤] R730.52[医药卫生—临床医学]