PLCL2基因多态性与大动脉粥样硬化型缺血性卒中的关系研究  

Study on relationship between PLCL2 gene polymorphism and large artery atherosclerotic ischemic stroke

作　　者：王晓蓓 李沛珊 吴勤奋 朱国峰[1,2] 杨新玲 WANG Xiaobei;LI Peishan;WU Qinfen;ZHU Guofeng;YANG Xinling(Department of Neurology,the Second Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830063,China;Xinjiang Clinical Research Center for Nervous System Diseases,Urumqi,Xinjiang 830063,China;Xinjiang Medical University,Urumqi,Xinjiang 830017,China)

机构地区：[1]新疆医科大学第二附属医院神经内科,乌鲁木齐830063 [2]新疆神经系统疾病临床研究中心,乌鲁木齐830063 [3]新疆医科大学,乌鲁木齐830017

出　　处：《重庆医学》2024年第14期2148-2153,共6页Chongqing medicine

基　　金：新疆维吾尔自治区自然科学基金项目(2021D01C376)。

摘　　要：目的探讨磷脂酶C样蛋白2(PLCL2)基因rs4535211、rs75885714、rs7653834位点单核苷酸多态性与大动脉粥样硬化(LAA)型缺血性卒中的相关性。方法选取2021年7月至2022年7月新疆医科大学第二附属医院就诊的105例新发LAA型缺血性卒中患者作为观察组,选取同期该院性别、年龄相匹配的103例体检者作为对照组。收集并比较两组临床资料及血清炎症指标,同时检测两组PLCL2基因rs4535211、rs75885714、rs7653834位点的基因型,并计算基因型和等位基因频率。结果观察组C反应蛋白(CRP)、白细胞介素-6(IL-6)、中性粒细胞与淋巴细胞比值(NLR)、单核细胞与高密度脂蛋白-胆固醇比值(MHR)、血小板与淋巴细胞比值(PLR)、D-二聚体水平高于对照组,高密度脂蛋白-胆固醇(HDL-C)水平低于对照组(P<0.05)。rs7653834位点为C/C、C/T、T/T基因型,两组比较差异有统计学意义(P<0.05)。两组rs7653834位点C/C、T/C、T/T基因型NLR、PLR水平比较,差异有统计学意义(P<0.05)。共显性模型、显性模型、超显性模型分析结果显示,两组rs7653834位点基因型比较,差异有统计学意义(P<0.05)。结论PLCL2基因rs7653834位点多态性与LAA型缺血性卒中可能存在潜在关联。Objective To study the correlation between single nucleotide polymorphisms at loci rs4535211,rs75885714,and rs7653834 of phosphatidylinositol-specific phospholipase 2(PLCL2)gene and large artery atherosclerotic(LAA)ischemic stroke.Methods A total of 105 patients with newly diagnosed LAA ischemic stroke admitted to the Second Affiliated Hospital of Xinjiang Medical University from July 2021 to July 2022 were selected as the observation group,and 103 patients with gender and age matching physical examination in this hospital during the same period were selected as the control group.The clinical data and serum inflammatory markers were collected and compared between the two groups.Genotypes of PLCL2 gene rs4535211,rs75885714 and rs7653834 loci in the two groups were detected,and genotype and allele frequencies were calculated.Results The levels of C-reactive protein(CRP),interleukin-6(IL-6),neutrophil to lymphocyte ratio(NLR),monocyte to high-density lipoprotein-cholesterol ratio(MHR),platelet to lymphocyte ratio(PLR)and D-dimer in the observation group were higher than those in the control group.The level of high density lipoprotein-cholesterol(HDL-C)was lower than that of the control group(P<0.05).rs7653834 locus was C/C,C/T,T/T genotypes,and the difference between the two groups was statistically significant(P<0.05).The levels of C/C,T/C and T/T genotypes NLR and PLR at rs7653834 locus were statistically significant between the two groups(P<0.05).The analysis results of co-dominant model,dominant model and overdominant model showed that there was statistical significance in rs7653834 locus genotype between the two groups(P<0.05).Conclusion There may be a potential association between rs7653834 locus polymorphism of PLCL2 gene and LAA type ischemic stroke.

关 键 词：磷脂酶C样蛋白2 基因多态性 大动脉粥样硬化型脑梗死 遗传模型 关系 

分 类 号：R743.3[医药卫生—神经病学与精神病学]