A signaling cascade mediating fruit trait development via phosphorylation-modulated nuclear accumulation of JAZ repressor  

作　　者：Wei Wang Jinyao Ouyang Yating Li Changsheng Zhai Bing He Huahan Si Kunsong Chen Jocelyn K.C.Rose Wensuo Jia 

机构地区：[1]College of Horticulture,China Agricultural University,Beijing 100193,China [2]College of Agriculture and Biotechnology,Zhejiang University,Zijingang Campus,Hangzhou 310058,China [3]Plant Biology Section,School of Integrative Plant Science,Cornell University,Ithaca14853,NY,USA [4]Institute of Horticulture Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830000,China

出　　处：《Journal of Integrative Plant Biology》2024年第6期1106-1125,共20页植物学报（英文版）

基　　金：financially supported by the National Key Research and Development Program(2022YFD2100102);the Tianchi Talent Program(2023),the Beijing Natural Science Foundation(6232019);the National Key Research and Development Program(2019YFD1000200);the Construction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects(CEFFPXM2019_014207_000032)。

摘　　要：It is generally accepted that jasmonate-ZIM domain(JAZ)repressors act to mediate jasmonate(JA)signaling via CORONATINE-INSENSITIVE1(COI1)-mediated degradation.Here,we report a cryptic signaling cascade where a JAZ repressor,FvJAZ12,mediates multiple signaling inputs via phosphorylation-modulated subcellular translocation rather than the COI1-mediated degradation mechanism in strawberry(Fragaria vesca).FvJAZ12 acts to regulate flavor metabolism and defense response,and was found to be the target of Fv MPK6,a mitogen-activated protein kinase that is capable of responding to multiple signal stimuli.FvMPK6 phosphorylates FvJAZ12 at the amino acid residues S179 and T183 adjacent to the PY residues,thereby attenuating its nuclear accumulation and relieving its repression for FvMYC2,which acts to control the expression of lipoxygenase 3(FvLOX3),an important gene involved in JA biosynthesis and a diverse array of cellular metabolisms.Our data reveal a previously unreported mechanism for JA signaling and decipher a signaling cascade that links multiple signaling inputs with fruit trait development.

关 键 词：defense response JAZ repressor PHOSPHORYLATION strawberry subcellular translocation 

分 类 号：Q943.2[生物学—植物学]