脱油油樟叶总酚提取纯化工艺优化及体外抗氧化研究  

Optimization of Extraction and Purification Process of Total Phenols from Deoiled Cinnamomum Longepaniculatum Leaves and Its Antioxidant Study in Vitro

作　　者：周希 蒋屿 袁叶飞 ZHOU Xi;JIANG Yu;YUAN Yefei(Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学,四川泸州646000

出　　处：《中国现代应用药学》2024年第11期1499-1507,共9页Chinese Journal of Modern Applied Pharmacy

基　　金：四川省科技计划项目(2022YFS0436);叙州区人民政府-西南医科大学科技战略合作项目(2021XZXNYD06)。

摘　　要：目的优选脱油油樟叶总酚的提取纯化工艺,考察纯化前后的抗氧化活性,为油樟合理开发和利用提供依据。方法在单因素试验基础上结合正交试验法,优选提取工艺;采用大孔吸附树脂法,优选纯化工艺;并采用DPPH、ABTS+法及总还原能力测定比较总酚纯化前后的体外抗氧化活性。结果总酚最佳提取工艺:液料比30 mL·g^(-1),乙醇浓度50%,回流时间2 h,提取温度90℃,提取2次,脱油油樟叶总酚提取量为15.63 mg·g^(-1)。最佳纯化工艺:采用HPD-600树脂,上样液浓度0.5 g·L^(-1),上样液pH 3,上样量2.5 BV,上样流速1 mL·min^(-1),4.5 BV蒸馏水除杂,乙醇洗脱浓度为60%,洗脱液pH 7,洗脱流速1 mL·min^(-1),收集4.5 BV洗脱液,总酚含量由12.51%提升至26.40%。纯化后总酚体外抗氧化能力良好,DPPH、ABTS+自由基清除能力IC50值分别为(38.07±0.66)mg·L^(-1)和(14.28±0.19)mg·L^(-1),总还原能力为纯化前的1.68倍。结论该工艺方法稳定可行,适用于工业生产。OBJECTIVE To explore the optimal process of extraction and purification of total phenols from deoiled Cinnamomum longepaniculatum leaves,and to evaluate the antioxidant activity in vitro before and after purification,so as to provide a theoretical basis for rational development and utilization of Cinnamomum longepaniculatum.METHODS The L18(37)orthogonal experiment was carried out on the basis of five-single factor and three-level experiments,such as concentration of ethanol(A),ratio of liquid-material(B),extraction temperature(C),extraction time(D),and extraction times(E).The extraction process of total phenols was optimized,by taking the amount of total phenols extraction as the process investigation index.Taking the adsorption and desorption performance under dynamic and static conditions as the investigation index of total phenols purification process,the purification process of total phenols was optimized by macroporous resin.With vitamin C(Vc)as the positive control,the antioxidant activity in vitro of total phenols before and after purification were evaluated by DPPH radical scavenging,ABTS+radical scavenging and determination of total reducing capacity.RESULTS The best extraction technology conditions of total phenols were as follows:ratio of liquid-material 30 mL·g^(-1),concentration of ethanol 50%,extraction time 2 h,extraction temperature 90℃,extraction 2 times.Under such conditions,the extraction amount of total phenols from deoiled Cinnamomum longepaniculatum leaves was 15.63 mL·g^(-1).HPD-600 type macroporous resin showed the best purifying profile.The best purification technology conditions were as follows:the concentration of sample solution was 0.5 g·L^(-1),the sample solution pH value was 3,the sample volume was 2.5 BV,the sample flow rate 1 mL·min^(-1),the impurity was removed by 4.5 BV distilled water,the elution concentration of ethanol was 60%,the eluate pH value 7,the elution flow rate 1 mL·min^(-1),4.5 BV eluent was collected and the total phenols assay was increased from 12.51%to 26

关 键 词：油樟叶 总酚 提取 纯化 大孔树脂 抗氧化 

分 类 号：R284.1[医药卫生—中药学]