MicroRNA-193a抑制β-catenin通路调控颞下颌关节骨关节炎炎症进展的机制研究  

MicroRNA-193a activatesβ-catenin pathway and regulates the progression of temporomandibular joint osteoarthritis

作　　者：程珏 孔令伟[1] 邱文风[1] 栗晓东 梁星星 程琳 辛鹏飞 CHENG Jue;KONG Ling-wei;QIU Wen-feng;LI Xiao-dong;LIANG Xing-xing;CHENG Lin;XIN Peng-fei(Beijing Jiaotong University Hospital,Beijing 100044,China;Shanxi Bethune Hospital(Shanxi Academy of Medical Sciences),Shanxi Taiyuan 030032,China)

机构地区：[1]北京交通大学医院,北京100044 [2]山西白求恩医院(山西医学科学院),山西太原030032

出　　处：《临床口腔医学杂志》2024年第6期323-327,共5页Journal of Clinical Stomatology

基　　金：山西省卫生计生委科研课题计划项目(2018017);山西省基础研究计划青年科学研究项目(编号:20210302124624)。

摘　　要：目的:探讨miR-193a调控颞下颌关节骨关节炎症进展的作用机制。方法:构建颞下颌关节炎大鼠模型,将大鼠根据转染情况分为Control、Model、miR-NC、miR-193a、miR-193a+LiCl(Wnt/β-catenin通路激活剂)组,检测大鼠疼痛学行为,RT-qPCR检测颞下颌关节组织miR-193a表达情况,Western blot检测β-catenin、基质金属蛋白酶、Nav1.7表达;ELISA检测炎症细胞因子;免疫组化检测CD68和iNOS表达。结果:过表达miR-193a可增加大鼠摆头阈值、IL-10表达水平,抑制β-catenin、基质金属蛋白酶、Nav1.7、TNF-α、IL-1β、IL-6、IL-23、CD68、iNOS表达水平(P<0.05)。激活β-catenin显著逆转了过表达miR-193a对上述指标的影响(P<0.05)。结论:过表达miR-193a可通过β-catenin减缓调控颞下颌关节骨关节炎症进展。Objective:To investigate the mechanism of miR-193a in regulating the progression of temporomandibular joint osteoarthritis.Methods:Rat model of temporomandipper arthritis was constructed,and the rats were divided into Control,Model,miR-NC,miR-193a and miR-193a+LiCl(Wnt/β-catenin pathway activator)groups according to the transfection conditions.The pain behaviors of the rats were examined,RT-qPCR was used to detect the expression of miR-193a in temporomandibular joint,and Western blot was used to detect the expression ofβ-catenin,matrix metalloproteinase and Nav1.7.Inflammatory cytokines were detected by ELISA.The expressions of CD68 and iNOS were detected by immunohistochemistry.Results:Overexpression of miR-193a increased the swing-head threshold,IL-10 expression level,and inhibited the expression levels ofβ-catenin,matrix metalloproteinase,Nav1.7,TNF-α,IL-1β,IL-6,IL-23,CD68 and iNOS in rats(P<0.05).Activation ofβ-catenin significantly reversed the effect of overexpression of miR-193a on the above indexes(P<0.05).Conclusion:Overexpression of miR-193a can reduce and control the progression of temporomandibular joint osteoarthritis throughβ-catenin.

关 键 词：MicroRNA-193a 颞下颌关节骨关节炎 Β-CATENIN 炎症反应 

分 类 号：R782.6[医药卫生—口腔医学]