食管鳞癌中microRNA let-7a-3甲基化与IGF-Ⅱ的相关性  

The correlation between microRNA let-7a-3 methylation and IGF-Ⅱexpression in esophageal squamous cell carcinoma

作　　者：朱公建[1] 郭红云[1] 白悦 王晓敏 朱小康[1] 刘东彦[1] 刘玉琴[1] ZHU Gongjian;GUO Hongyun;BAI Yue;WANG Xiaomin;ZHU Xiaokang;LIU Dongyan;LIU Yuqin(Department of Science and Education,Gansu Provincial Academy of Medical Science/Sun Yat-sen University Cancer Center Gansu Hospital,Lanzhou 730050,China;Department of Gastroenterology,Xigu District of Lanzhou City People′s Hospital)

机构地区：[1]甘肃省医学科学研究院/中山大学附属肿瘤医院甘肃医院科教科,兰州730050 [2]兰州市西固区人民医院消化内科

出　　处：《实用肿瘤学杂志》2024年第3期173-178,共6页Practical Oncology Journal

基　　金：兰州市人才创业创新项目(编号:2018-RC-96)。

摘　　要：目的探讨食管鳞癌组织中microRNA let-7a-3甲基化状态与血浆中类胰岛素样生长因子2(Insulin like growth factor 2,IGF-Ⅱ)表达的相关性。方法采用甲基化特异性PCR法(Methylation specific PCR,qMSP)检测83例食管癌及相对应的癌旁正常组织中let-7a-3甲基化状态,采用酶联免疫吸附试验(Enzyme linked immunosorbent assay,ELISA)检测血浆中IGF-Ⅱ的表达水平。结果83例食管鳞癌患者癌组织中的microRNA let-7a-3甲基化程度显著高于癌旁正常组织(P<0.001)。83例食管鳞癌患者血浆中IGF-Ⅱ的表达水平与let-7a-3基因的甲基化程度总体上呈正相关,具有统计学意义(r=0.600,P<0.001)。结论microRNA let-7a-3可能通过对下游分子的甲基化调控参与食管鳞癌的发生发展,这对了解食管鳞癌形成的机制具有重要意义,可为食管鳞癌的诊断和预后提供依据。Objective The aim of this study was to explore the correlation between the methylation status of microRNA let-7a-3 in esophageal squamous cell carcinoma(ESCC)and the expression of insulin-like growth factor 2(IGF-Ⅱ).Methods The methylation specific PCR(qMSP)was used to detect the methylation status of let-7a-3 in 83 cases of esophageal cancer and corresponding adjacent normal tissues.The enzyme linked immunosorbent assay(ELISA)was used to detect the expression of IGF-II in plasma.Results The degree of let-7a-3 methylation in cancer tissues of 83 patients with ESCC was significantly higher than that in normal tissues adjacent to cancer(P<0.001).The expression of IGF-Ⅱin the plasma of 83 patients with ESCC was positively correlated with the methylation degree of let-7a-3,which was statistically significant(r=0.600,P<0.001).Conclusion microRNA let-7a-3 may participate in the occurrence and progression of ESCC by regulating the methylation of downstream molecules,which is of great significance for understanding the mechanisms of ESCC development and providing a basis for the diagnosis and prognosis of ESCC.

关 键 词：食管鳞癌 let-7a-3 甲基化 类胰岛素样生长因子2 

分 类 号：R735.1[医药卫生—肿瘤]