重度抑郁症患者外泌体miR-146a-5p表达对脑灰质容积变化的调控作用  

Regulation of exosomal miR-146a-5p expression on gray matter volume changes in patients with major depressive disorder

作　　者：苏婕 刘莎[2] 史长洲 张萱萱 郑青彤 李博 邓燕佳 王兴田 文戈[5] 刘恺 SU Jie;LIU Sha;SHI Changzhou;ZHANG Xuanxuan;ZHENG Qingtong;LI Bo;DENG Yanjia;WANG Xingtian;WEN Ge;LIU Kai(Department of Radiology,the Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu Province 221000,China;the Affiliated Brain Hospital of Guangzhou Medical University,Guangzhou 510370,China;School of Medical Imaging,Xuzhou Medical University,Xuzhou,Jiangsu Province 221000,China;Department of Ultrasound Medicine,the Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu Province 221000,China;Imaging Center,Nanfang Hospital,Southern Medical University,Guangzhou 516006,China)

机构地区：[1]徐州医科大学附属医院影像科,江苏徐州221000 [2]广州医科大学附属脑科医院,广东广州510370 [3]徐州医科大学影像学院,江苏徐州221000 [4]徐州医科大学附属医院超声医学科,江苏徐州221000 [5]南方医科大学南方医院影像中心,广东广州516006

出　　处：《实用放射学杂志》2024年第6期861-865,共5页Journal of Practical Radiology

基　　金：国家自然科学基金面上项目(82271980);徐州市重点研发计划(社会发展)项目(KC22124)。

摘　　要：目的探讨外泌体miR-146a-5p表达对重度抑郁症(MDD)患者脑灰质容积的影响。方法选取113例MDD患者(MDD组)及107例健康对照(HC)(HC组),收集外周血并提取外泌体,测定miR-146a-5p表达,同时利用MR扫描获得MDD及HC的颅脑高分辨率T1WI图像,使用SPM12软件进行处理,计算脑灰质容积,并利用SPM的Flexible factorial设计分析“抑郁×miR-146a-5p表达”2个因素对脑灰质容积的交互效应,并通过平均值评估组间差别,继而分析MDD相关的脑灰质容积异常是否依赖于miR-146a-5p表达。结果相较于HC组,MDD组外泌体miR-146a-5p表达显著升高;此外,基于体素的析因分析结果发现,MDD组中miR-146a-5p高表达与前、后扣带回灰质体积减小存在关联(独立体素阈值P<0.001,AlphaSim矫正),且2处脑区灰质容积较HC组显著减低。结论外泌体miR-146a-5p在MDD患者中存在过表达,并且其与MDD患者特定区域脑皮层萎缩存在关联。Objective To investigate the effect of exosomal miR-146a-5p expression on gray matter volume in patients with major depressive disorder(MDD).Methods A total of 113 MDD patients(MDD group)and 107 healthy controls(HC)(HC group)were selected.Peripheral blood was collected and exosomes were isolated to quantify miR-146a-5p expression.Brain high-resolution T1WI images of MDD and HC were obtained via MR,and gray matter volume was computed via SPM12 software.The interaction effect of“Depression×miR-146a-5p expression”on gray matter volume was analyzed using SPM’s Flexible factorial design,and the between-group difference was assessed by extracting the mean value,thus to analyze whether MDD-related gray matter volume abnormalities were dependent on miR-146a-5p expression.Results Exosomal miR-146a-5p expression was significantly elevated in MDD group compared to HC group.Voxel-based factorial analysis revealed a relationship between high miR-146a-5p expression in MDD group and reduced gray matter volume in the anterior and posterior cingulate cortices(independent voxel threshold P<0.001,AlphaSim corrected),and a significantly reduced gray matter volume as compared with HC group was detected in the two regions.Conclusion The exosomal miR-146a-5p is overexpressed in patients with MDD and may be associated with specific cortical atrophy in patients with MDD.

关 键 词：抑郁症 外泌体 miR-146a-5p 磁共振成像 灰质容积 

分 类 号：R749.4[医药卫生—神经病学与精神病学] R445.2[医药卫生—临床医学]