抗黑色素瘤分化相关蛋白5与抗Ro52阳性/阴性患者的临床特征及KL-6水平变化及意义  

Clinical characteristics and KL-6 level changes and significance of anti melanoma differentiation related protein 5 and anti-Ro52 positive/negative patient

作　　者：刘轩[1] 王桂蓉[1] 李鸿斌[1] LIU Xuan;WANG Guirong;LI Hongbin(Rheumatology Laboratory of Inner Mongolia Medical University Affiliated Hospital,Huhhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院风湿科实验室,内蒙古呼和浩特010050

出　　处：《国际检验医学杂志》2024年第S01期4-7,共4页International Journal of Laboratory Medicine

基　　金：内蒙古医科大学科技百万工程联合项目〔YKD2018KJBW(LH)021〕。

摘　　要：目的研究抗黑色素瘤分化相关蛋白5(anti-MDA5)抗体与抗Ro抗体-52KD(anti-Ro52)抗体阳性或anti-Ro52抗体阴性患者的临床特征和血清涎液化糖链抗原-6(KL-6)水平变化。方法选取2018年1月至2021年11月申请肌炎自身抗体检测的患者样本470例,采用线性免疫印迹法(LIA)筛选出anti-MDA5抗体阳性50例,分为anti-Ro52抗体阳性(anti-Ro52+)组与anti-Ro52抗体阴性(anti-Ro52-)组,化学发光酶免疫分析法(CLEIA)测定两组患者的血清KL 6水平并比较两组患者的临床资料。结果anti-MDA5抗体阳性患者的病程越短,死亡率越高;anti-MDA5抗体阳性患者最常见的临床表现为皮肤病变,如皮肤瘙痒、皮疹和皮肤溃疡,anti-Ro52+组发生率为93.75%,anti-Ro52-组发生率为94.44%;anti-MDA5抗体阳性anti-Ro52+组乙肝患病率(12.5%)低于anti-Ro52-组(66.67%);anti-MDA5抗体阳性anti-Ro52+组间质性肺病(ILD)的发生率(84.38%)高于anti-Ro52-组(55.56%);anti-Ro52+组外周血KL-6水平(1211.25±590.13 U/mL)也明显高于anti-Ro52-组(713.63±468.62 U/mL),差异有统计学意义(P<0.05)。结论anti-MDA5抗体和anti-Ro52抗体是ILD患者的危险因素,两种抗体共存的患者更易发生ILD,且外周血KL-6水平明显高于anti-Ro52抗体阴性的患者。Objective To investigate the clinical characteristics and changes in serum salivary liquefied gly-can antigen-6(KL-6)levels in patients with positive or negative anti-Ro52 antibodies against melanoma differ-entiation associated protein 5(anti-MDA5)and anti-Ro antibody 52KD(anti-Ro52).Methods A sample of 470 patients who applied for myositis autoantibody testing from January 2018 to November 2021 was selected.Linear immunoblotting(LIA)was used to screen for 50 anti-MDA5 antibody positive cases,which were divid-ed into anti-Ro52 antibody positive(anti-Ro52+)group and anti-Ro52 antibody negative(anti-Ro52-)group.The serum KL6 levels of the two groups of patients were measured by chemiluminescence enzyme immunoas-say(CLEIA),and the clinical data of the two groups of patients were compared.Results The shorter the course of illness,the higher the mortality rate of anti-MDA5 antibody positive patients;The most common clinical manifestations of anti-MDA5 antibody positive patients are skin lesions,such as skin itching,rash,and skin ulcers.The incidence rate of anti-Ro52+group is 93.75%,and the incidence rate of anti-Ro52-group is 94.44%;The prevalence of hepatitis B in anti MDA5 antibody positive anti Ro52+group(12.5%)was lower than that in anti Ro52-group(66.67%);The incidence of interstitial lung disease(ILD)in the anti-MDA5 antibody positive anti-Ro52+group(84.38%)was higher than that in the anti-Ro52-group(55.56%);The peripheral blood KL-6 level in the anti-Ro52+group(1211.25±590.13 U/mL)was also significantly higher than that in the anti-Ro52-group(713.63±468.62 U/mL),and the difference was statistically significant(P<005).Conclusion Anti-MDA5 antibody and anti-Ro52 antibody are risk factors for ILD patients.Patients with coexistence of the two antibodies are more likely to develop ILD,and their peripheral blood KL-6 levels are significantly higher than those with anti-Ro52 antibody negativity.

关 键 词：anti-MDA5抗体 anti-Ro52抗体 KL-6 临床特征 

分 类 号：R739.5[医药卫生—肿瘤] R446[医药卫生—临床医学]