氯化锶与生物活性玻璃协同作用对牙本质再矿化的体外研究  

In vitro study of dentin remineralization by synergistic action of strontium chloride and bioactive glass

作　　者：牛宇庭 何丽娜[1] 李艳萍 朱昊 陈佳媛 陈佳欣 张琳[1] NIU Yuting;HE Lina;LI Yanping;ZHU Hao;CHEN Jiayuan;CHEN Jiaxin;ZHANG Lin(Department of Endodontics,The First Hospital of Harbin Medical University,Harbin 150007)

机构地区：[1]哈尔滨医科大学附属第一医院,150007

出　　处：《现代口腔医学杂志》2024年第3期183-188,共6页Journal of Modern Stomatology

摘　　要：目的体外研究氯化锶与生物活性玻璃协同作用对牙本质再矿化的影响。方法选择44颗人离体上颌第三磨牙,制备牙本质敏感模型(32个方形+12个类圆形),随机分为四组进行不同处理(n=11),分别为对照组(蒸馏水)、氯化锶组、生物活性玻璃组、氯化锶+生物活性玻璃组。7天后,类圆形样本用X射线衍射(XRD)对再矿化物进行定性分析;方形样本每组取4个用扫描电镜(SEM)观察其表面及纵断面牙本质小管封闭情况、X射线能谱(EDX)分析再矿化物的主要元素组成及钙磷比;每组剩余4个方形样本经6%柠檬酸酸蚀后用扫描电镜(SEM)观察其表面及纵断面牙本质小管封闭情况以评估再矿化物的耐酸性。结果氯化锶、生物活性玻璃以及氯化锶+生物活性玻璃组牙本质小管均有不同程度的封闭,其中氯化锶+生物活性玻璃组牙本质小管封闭率(90.72%)显著高于生物活性玻璃组(80.71%)及氯化锶组(55.38%),除对照组外,各组牙本质小管内均可见再矿化物。酸处理后各组牙本质小管有不同程度的开放、封闭率下降,其中氯化锶+生物活性玻璃组及生物活性玻璃组牙本质小管封闭率分别为66.67%、58.66%,高于氯化锶组(41.58%),除对照组外,各组牙本质小管内仍可见再矿化物。X射线能谱分析显示各组检测出的主要元素为钙、磷、氧,氯化锶组及氯化锶+生物活性玻璃组中还有锶,各组间钙磷比差异无统计学意义(P>0.05)。X射线衍射结果表明氯化锶及氯化锶+生物活性玻璃组再矿化物均出现锶羟基磷灰石及羟基磷灰石特征性衍射峰,生物活性玻璃组再矿化物出现羟基磷灰石特征性衍射峰。结论氯化锶与生物活性玻璃协同作用显著提高牙本质小管封闭率,所形成再矿化物的主要元素组成及钙磷比与天然牙本质相似;氯化锶+生物活性玻璃组及生物活性玻璃组对酸的抵抗力相同,且优于氯化锶组。Objective To study the effect of synergistic action of strontium chloride and bioactive glass on dentin remineralization in vitro.Methods Forty-four human isolated maxillary third molars were selected,and dentin sensitivity models(32 squares+12 analogous rounds)were prepared and randomly divided into four groups for different treatments(n=11),namely,control(distilled water),strontium chloride,bioactive glass,and strontium chloride+bioactive glass groups.7 days later,analogous rounds samples were used for the characterization of remineralizers by X-ray diffraction(XRD).Four square samples from each group were analyzed by scanning electron microscopy(SEM)to observe the sealing of dentin tubules on their surfaces and vertical section,and by X-ray spectroscopy(EDX)to analyze the major elemental compositions and calcium to phosphorus ratios of the remineralized substances.The remaining four square samples from each group were etched with 6%citric acid and then scanned by scanning electron microscopy(SEM)to observe the sealing of dentin tubules on their surfaces and vertical section in order to evaluate the acid resistance of the remineralized substances.Results Dentin tubules were closed to different degrees in the strontium chloride,bioactive glass,and strontium chloride+bioactive glass groups,with the rate of dentin tubule closure in the strontium chloride+bioactive glass group(90.72%),which was significantly higher than that in the bioactive glass group(80.71%)and the strontium chloride group(55.38%).Except for the control group,remineralized substances were found in dentin tubules of each group.There were different degrees of opening and decrease in the closure rate of dentin tubules in each group after acid treatment.The closure rate of dentin tubules in the strontium chloride+bioactive glass group and bioactive glass group were 66.67%and 58.66%,respectively,which were higher than that in the strontium chloride group(41.58%).Except for the control group,remineralized substances were still found in dentin tubules

关 键 词：牙本质再矿化 生物活性玻璃 氯化锶 牙本质小管封闭率 钙磷比 耐酸性 

分 类 号：R780.2[医药卫生—口腔医学]