饲料中添加辅酶Q10对大口黑鲈生长、体组成、抗氧化能力和抗低氧胁迫的影响  

Effects of Coenzyme Q10 Supplementation on Growth,Body Composition,Antioxidant Capacity and Hypoxia Stress of Largemouth Bass(Micropterus salmoides)

作　　者：舒斌 李建东 张磊 吕小燕 陈颖 吴坤岚 龚洋洋 SHU Bin;LI Jiandong;ZHANG Lei;LYU Xiaoyan;CHEN Ying;WU Kunlan;GONG Yangyang(Zhejiang NHU Co.,Ltd.,Zhejiang Shaoxing 312500,China)

机构地区：[1]浙江新和成股份有限公司,浙江绍兴312500

出　　处：《饲料工业》2024年第14期56-62,共7页Feed Industry

摘　　要：实验旨在研究饲料中添加辅酶Q10对大口黑鲈生长、体组成、抗氧化能力,以及在低氧应激下血清、肝脏抗氧化酶活性的影响,实验设计3种等氮等脂饲料,分别为400 g/kg鱼粉对照组(F0)、200 g/kg鱼粉对照组(F1)、200 g/kg鱼粉+50 mg/kg辅酶Q10(F2),以初始体重为(5.00±0.02)g的大口黑鲈为研究对象,进行为期56 d的养殖实验,实验结束1周后进行3~4 mg/L的低氧应激,并取样分析。结果表明:相比F1对照组,饲料中添加50 mg/kg辅酶Q10的增重率(WG)、特定生长率(SGR)和末均重(FW)显著提高(P<0.05);体成分中添加50 mg/kg辅酶Q10的粗蛋白含量最高,全鱼水分和粗脂肪含量各组无显著差异(P>0.05);添加辅酶Q10血清谷草转氨酶(AST)的活性显著降低(P<0.05),其中50 mg/kg辅酶Q10组血清谷胱甘肽过氧化物(GSH-Px)、谷胱甘肽(GSH)和过氧化氢酶(CAT)活性均有提高(P>0.05)。饲料中添加50 mg/kg辅酶Q10的血清丙二醛(MDA)含量显著低于高鱼粉对照F0组(P<0.05)。在低氧应激条件下,添加辅酶Q10的血清GSH-Px和CAT活性相比F0组显著提高(P<0.05),而肝脏中相比其他实验组添加50 mg/kg辅酶Q10的GSH-Px和CAT活性最高。皮质醇含量50 mg/kg辅酶Q10相比高鱼粉对照F0组显著下降(P<0.05)。综上所述,饲料中添加50 mg/kg辅酶Q10能够促进大口黑鲈的生长性能,提高机体的抗氧化能力和低氧应激下的抗胁迫能力。The aim was to investigate the effect of feed supplementation with coenzyme Q10 on Microp-terus Salmoides growth,body composition,antioxidant capacity,and serum and liver antioxidant enzyme activity under low oxygen stress.Three isonitrogenous and isoenergetic diets were formulated,400g/kg fish meal control(F0),200 g/kg fish meal control(F1),200 g/kg fish meal+50 mg/kg coenzyme Q10(F2).The diets were fed to M.salmoides with the initial body weight of(5.00±0.02)g for 56 daysHypoxic stress at 3-4 mg/L was performed 1 week after the end of the experiment and sampled for analysis.The results showed as follows:Compared with the control group F1,WG,SGR and FW supplemented with 50mg/kg coenzyme Q10 were significantly increased(P<0.05).The highest crude protein was 50 mg/kg coenzyme Q10.Fish water and crude fat content were not significantly different between groups(P>0.05).The serum of AST supplemented with coenzyme Q10 was significantly decreased(P<0.05).The serum GSH-Px,GSH and CAT activities in the 50 mg/kg coenzyme Q10 group were higher than the control group F1(P>0.05),and the serum MDA content was significantly lower than the high fish meal control group F0(P<0.05).Under low-oxygen stress conditions,significantly increased serum GSH-Px and CAT activity supplemented with co-enzyme Q10(P<0.05),GSH-Px and CAT activity with 50 mg/kg coenzyme Q10 were the highest in liver compared with other experimental groups.The cortisol content of 50 mg/kg Coenzyme Q10 was signifi-cantly lower than that of F0 group(P<0.05).In conclusion,the addition of 50 mg/kg Coenzyme Q10 to the feed can promote the growth performance of M.salmoides,improve the antioxidant capacity of the body and the anti stress capacity under hypoxia stress.

关 键 词：辅酶Q10 生长 体组成 抗氧化能力 低氧胁迫 大口黑鲈 

分 类 号：S816.32[农业科学—饲料科学]