流式细胞术检测皮肤组织在母细胞性浆细胞样树突细胞肿瘤诊断中的应用  

Application of cutaneous tissue detected by flow cytometry in the diagnosis of blastic plasmacytoid dendritic cell neoplasm

作　　者：郑凤丽 王春艳 刘松雅 毛霞 易淑娟 ZHENG Feng-li;WANG Chun-yan;LIU Song-ya;MAO Xia;YI Shu-juan(Department of Hematology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Wuhan 430030,China;Department of Hematology,Shanxi Bethune Hospital,Shanxi Academy of Medical Sciences,Tongji Shanxi Hospital,Shanxi Taiyuan 030032,China)

机构地区：[1]华中科技大学同济医学院附属同济医院血液内科,湖北武汉430030 [2]山西白求恩医院(山西医学科学院同济山西医院)血液内科,山西太原030032

出　　处：《内科急危重症杂志》2024年第3期224-228,共5页Journal of Critical Care In Internal Medicine

摘　　要：目的:探讨流式细胞术(FCM)检测皮肤组织样本在诊断母细胞性浆细胞样树突细胞肿瘤(BPDCN)中的作用。方法:利用多色流式检测皮肤组织与骨髓,并结合组织病理学、骨髓细胞形态学、遗传学、二代测序(NGS)等检测结果对3例已确诊的BPDCN进行回顾性分析。结果:3例患者均以皮肤损害为首发症状,FCM检测骨髓仅发现1例患者标本存在BPDCN细胞,而3例患者皮肤组织标本中均发现BPDCN细胞;与皮肤组织病理免疫组化结果诊断一致;病例2患者骨髓细胞学和遗传学检查均未发现BPDCN细胞,病例1患者骨髓细胞学中发现BPDCN细胞,病例3患者NGS检测到BPDCN相关基因突变。结论:流式细胞免疫分型是BPDCN诊断的实用辅助手段,可以在早期皮肤组织标本中快速精准地诊断BPDCN,同时进行鉴别诊断。Objective:To investigate the value of flow cytometry(FCM)in the diagnosis of blastic plasmacytoid dendritic cell neoplasm(BPDCN)by detecting cutaneous tissue samples.Methods:The cutaneous tissue and bone marrow were detected by multi-color FCM,and the results of histopathology,bone marrow cell morphology,genetics,and second-generation sequencing were analyzed retrospectively in 3 cases of confirmed BPDCN.Results:All 3 patients had skin damage as the initial symptom.FCM detection of bone marrow only found BPDCN cells in one patient sample,while FMC detection of skin tissue samples found BPDCN cells in all 3 patients.The diagnosis was consistent with the pathological and immunohistochemical diagnosis of skin tissue.The bone marrow cytology and genetic examination in one patient(case 2)did not detect BPDCN cells.BPDCN cells were found in bone marrow cytology of one patient(case 1),and BPDCN related gene mutations were detected in one patient(case 3)by second-generation sequencing.Conclusion:FCM immunophenotyping is a practical auxiliary tool for the diagnosis of BPDCN,which can quickly and accurately diagnose BPDCN in early skin tissue specimens,and perform differential diagnosis.It is an effective pathological auxiliary tool.

关 键 词：流式细胞术 皮肤 母细胞性浆细胞样树突细胞肿瘤 

分 类 号：R733.3[医药卫生—肿瘤]