响应面法优化天仙果总黄酮提取工艺及抗氧化活性研究  

Optimization of the Extraction Process of Total Flavonoids from Ficus erecta by Response Surface Methodology and Its Antioxidant Activity

作　　者：黄雅瑜 林米妮 蔡树芸 杨光乐 张秋萍[3] 张怡评 HUANG Ya-yu;LIN Mi-ni;CAI Shu-yun;YANG Guang-le;ZHANG Qiu-ping;ZHANG Yi-ping(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Technology Innovation Center for Exploitation of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,China;Xiamen Hospital of Traditional Chinese Medicine,Xiamen 361009,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]自然资源部第三海洋研究所海洋生物资源开发利用工程技术创新中心,福建厦门361005 [3]厦门市中医院,福建厦门361009

出　　处：《中国现代中药》2024年第7期1219-1226,共8页Modern Chinese Medicine

基　　金：福建省科技计划项目(2021N0029);厦门市科技计划项目(3502Z20227107)。

摘　　要：目的:优化天仙果总黄酮提取工艺,并测定其抗氧化活性。方法:通过响应面优化建立数学模型,考察乙醇体积分数、料液比、提取时间、提取次数4个因素,得到天仙果总黄酮最优提取条件。考察天仙果抗氧化活性,进行1,1-二苯基-2-三硝基苯肼(DPPH)法、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)法、羟自由基清除能力的测定。结果:天仙果总黄酮最佳提取工艺为乙醇体积分数60%、料液比1∶23、提取时间18 min、提取1次,在此条件下,提取率为16.25 mg·g^(-1),天仙果总黄酮对DPPH、ABTS、羟自由基清除率分别为92.84%、89.34%、69.73%。结论:优化后的提取工艺可操作性强、稳定可靠,天仙果抗氧化活性好。Objective:To optimize the extraction process and determine the antioxidant activity of total flavonoids from Ficus erecta(Thunb.)var.beecheyana(Hook.et Arn.)King.Methods:Four factors,namely ethanol concentration,material-liquid ratio,extraction time,and number of extractions,were selected to obtain the optimal conditions through response surface methodology and mathematical modeling,thus improving the extraction rate of total flavonoids from F.erecta.The in vitro antioxidant activity was investigated,measuring 1,1-Diphenyl-2-picrylhydrazyl radical(DPPH),2,2-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS),and hydroxyl radical scavenging capacities.Results:The optimal extraction process for total flavonoids from F.erecta was identified with 60%ethanol,1:23 material-liquid ratio,and 18-min extraction time,under which the extraction rate was 16.25 mg·g^(-1).The scavenging rates of total flavonoids from F.erecta for DPPH,ABTS,and hydroxyl radicals reached 92.84%,89.34%,and 69.73%,respectively.Conclusion:The optimized extraction process was operable,stable,and reliable,exhibiting good antioxidant activity.

关 键 词：天仙果 总黄酮 提取工艺 响应面法 抗氧化活性 

分 类 号：R284[医药卫生—中药学]