人类白细胞抗原基因分型方法分辨水平的专家共识  

Expert consensus on the resolution level of the methods for HLA genotyping

作　　者：中国血小板基因数据库协作组 浙江省血液中心 和艳敏[2] 毛伟[3] 李晓丰[4] 沈捷[5] 刘铮[6] 朱发明[2] Workshop of Blood Donor Database for Platelet Matching Transfusion in China,Blood Center of Zhejiang Province

机构地区：[1]不详 [2]浙江省血液中心 [3]重庆市血液中心 [4]辽宁省血液中心 [5]江苏省人民医院 [6]河南省红十字血液中心

出　　处：《中国输血杂志》2024年第7期721-727,共7页Chinese Journal of Blood Transfusion

摘　　要：人类白细胞抗原(human leukocyte antigen,HLA)基因分型已广泛应用于造血干细胞捐献者资料库建立、供受者组织配型、血小板基因数据库建立以及疾病关联诊断、遗传学和其他科学研究。随着HLA等位基因数量的增加和分型技术发展,对于HLA基因分型分辨水平界定、供受者HLA位点结果描述和配合判定等存在一定差异,为提高对HLA基因分型分辨水平的界定和解读的规范性,HLA基因检测实验室和临床移植领域的多名专家,依据国内外相关文献和临床实践制定此专家共识,对国内HLA基因分型分辨水平的定义、分型方法、供受者结果描述和相合判定等进行了概述并达成共识,以期进一步规范实验室HLA基因分型,保障检测结果的准确性。Human leukocyte antigen(HLA)genotyping has been widely used in establishing the database of the donors for hematopoietic stem cell,HLA matching selection between the donor and recipient,establishing the database of platelet donors with known HLA genotype,diagnosis of HLA association with diseases,genetics study and other scientific research.With the increasing number of HLA alleles and the development of HLA genotyping technology,there are some controversy in the definition of resolution for HLA genotyping,the description of the results for HLA genotyping between donor and recipient and the determination of HLA matching level.In order to improve the normalization of the definition and interpretation of HLA genotyping resolution,this expert consensus is formulated by many experts from the fields of HLA genotyping in the laboratory and clinical transplantation according to the relevant domestic and foreign literature and clinical practice.The definition of the resolution of HLA genotyping,the methods of HLA genotyping,the description of the results between donor and recipient and HLA matching determination are summarized,which will further standardize HLA genotyping technology and ensure the accuracy of the results for HLA genotyping.

关 键 词：人类白细胞抗原 等位基因 基因分型方法 分辨水平 描述方式 

分 类 号：R457.1[医药卫生—治疗学] R331.142[医药卫生—临床医学]