胸腺瘤组织Slug、TdT、SOX9蛋白表达与临床病理特征及预后的关系分析  

Analysis of the Relationship between Protein Expression of Slug,TdT and SOX9 and Clinicopathological Features and Prognosis in Thymoma Tissues

作　　者：王晓敏 陈亚龙 雷光焰 李林[1] 晏俊 王祥 丁彩霞 WANG Xiao-min;CHEN Ya-long;LEI Guang-yan;LI Lin;YAN Jun;WANG Xiang;DING Cai-xia(Department of Pathology,Shaanxi Cancer Hospital Affiliated to Medical College of Xi'an Jiaotong University,Xi'an,Shaanxi,710061,China;Department of Clinical Laboratory,Joint Logistics Support Force Lintong Rehabilitation and Rehabilitation Center,Xi'an,Shaanxi,710600,China;Department of Thoracic Surgery,Shaanxi Cancer Hospital Affiliated to Medical College of Xi'an Jiaotong University,Xi'an,Shaanxi,710061,China)

机构地区：[1]西安交通大学医学院附属陕西省肿瘤医院病理科,陕西西安710061 [2]联勤保障部队临潼康复疗养中心检验科,陕西西安710600 [3]西安交通大学医学院附属陕西省肿瘤医院胸外科,陕西西安710061

出　　处：《现代生物医学进展》2024年第11期2062-2067,共6页Progress in Modern Biomedicine

基　　金：陕西省自然科学基金项目(2018JM7028)。

摘　　要：目的:分析锌指转录因子(Slug)、末端脱氧核糖核苷酸转移酶(TdT)、Y染色体性别决定区相关高速泳动族框因子9(SOX9)蛋白表达与胸腺瘤患者临床病理特征及预后的关系。方法:选择2016年5月至2018年11月在本院接受手术切除的113例胸腺瘤组织及癌旁组织,利用免疫组织化学法测定Slug、TdT、SOX9蛋白阳性表达,分析Slug、TdT、SOX9蛋白表达与胸腺瘤患者临床病理特征、预后的关系。结果:胸腺瘤组织Slug、TdT、SOX9蛋白阳性表达率均高于癌旁组织(P<0.05);胸腺瘤组织Slug、Td T、SOX9蛋白阳性表达在患者年龄、性别、肿瘤直径、是否合并重症肌无力、是否伴有大血管侵犯方面比较,差异无统计学意义(P>0.05);胸腺瘤组织Slug、TdT、SOX9蛋白阳性表达在Masaoka-Koga分期、WHO分型方面比较,差异有统计学意义(P<0.05);胸腺瘤组织Slug、TdT、SOX9蛋白阳性表达与Masaoka-Koga分期、WHO分型呈正相关(P<0.05);113例胸腺瘤患者随访5年总生存率为81.42%(92/113),Slug阳性组5年生存率低于Slug阴性组(70.18%VS 92.86%),TdT阳性组5年生存率低于TdT阴性组(79.790%VS 89.57%),SOX9阳性组5年生存率低于SOX9阴性组(79.35%VS 90.48%)(χ^(2)=9.669、9.515、17.008,P<0.05);Cox比例风险回归模型分析发现,Masaoka-Koga分期(Ⅲ期/Ⅳ期)、WHO分型(B2/B3)、Slug蛋白阳性表达、TdT蛋白阳性表达、SOX9蛋白阳性表达是影响胸腺瘤患者预后的独立危险因素(HR:3.518、2.921、4.536、1.402、2.921,P<0.05)。结论:胸腺瘤组织Slug、TdT、SOX9呈高表达,三者蛋白表达与患者Masaoka-Koga分期、WHO分型有关,且是影响胸腺瘤患者5年生存率的独立危险因素。Objective:The relationship between the expression of zinc finger transcription factor(Slug),terminal deoxyribonucleotidyl transferase(TdT),Y chromosome sex determination region related high-speed swimming frame factor 9(SOX9)protein and clinicopathological features and prognosis of thymoma patients was analyzed.Methods:113 cases of thymoma tissues and adjacent tissues surgically resected in our hospital were selected from May 2016 to November 2018.The positive expression of Slug,TdT and SOX9 protein was determined by immunohistochemistry.The relationship between the expression of Slug,TdT and SOX9 protein and the clinicopathological features and prognosis of thymoma patients was analyzed.Results:The positive expression rates of Slug,TdT and SOX9 protein in thymoma tissues were higher than those in adjacent tissues(P<0.05).There was no significant difference in the positive expression of Slug,TdT and SOX9 protein in thymoma tissues in terms of age,gender,tumor diameter,whether combined with myasthenia gravis and whether accompanied by macrovascular invasion(P>0.05).The positive expression of Slug,TdT and SOX9 protein in thymoma tissues was significantly different in Masaoka-Koga stage and WHO classification(P<0.05).The positive expression of Slug,TdT and SOX9 protein in thymoma tissues was positively correlated with Masaoka-Koga stage and WHO classification(P<0.05).The 5-year overall survival rate in 113 thymoma patients was 81.42%(92/113),the 5-year survival rate in Slug positive group was lower than that in Slug negative group (70.18% VS 92.86%). the 5-year survival rate in TdT positive group was lower than that in TdT negativegroup (79.790% VS 89.57%), the 5-year survival rate in SOX9 positive group was lower than that in SOX9 negative group (79.35% VS90.48% ) (χ^(2)=9.669, 9.515, 17.008, P<0.05). Cox proportional hazard regression model analysis showed that, Masaoka-Koga stage(stageIII/stageIV), WHO classification (B2/B3), positive expression of Slug protein, positive expression of TdT protein and positiveexp

关 键 词：胸腺瘤 SLUG TDT SOX9 临床病理特征 预后 

分 类 号：R734.5[医药卫生—肿瘤]