TE融合基因调控Wnt/β-catenin通路促进前列腺癌细胞转移  

TE fusion gene promotes the metastasis of prostate cancer cells by regulating Wnt/β-catenin pathway

作　　者：郝晓明 王玉杰[1] HAO Xiaoming;WANG Yujie(Department of Urology,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院泌尿外科,新疆乌鲁木齐830054

出　　处：《生物技术》2024年第3期348-352,共5页Biotechnology

摘　　要：[目的]研究TE融合基因对前列腺癌DU145细胞转移的影响。[方法]将前列腺癌DU145细胞随机分为3个实验组:siRNA NC实验组、siRNA T2E实验组与KYA1797K实验组。采用MTT实验检测DU145细胞的增殖速度,Transwell实验检测DU145细胞的侵袭能力,流式细胞术检测DU145细胞的凋亡率,蛋白免疫印迹方法分析DU145细胞中Wnt/β-catenin通路蛋白的表达。[结果]与siRNA NC实验组比较,siRNA T2E以及KYA1797K实验组的DU145细胞增殖能力下降(1.73±0.35 vs 1.02±0.22 vs 0.91±0.27,P<0.05);siRNA T2E以及KYA1797K实验组的DU145细胞侵袭数量下降(167.28±12.89 vs 78.21±9.26 vs 82.33±10.33,P<0.05);siRNA T2E以及KYA1797K实验组的DU145细胞凋亡率增加(8.25%±0.89%vs 28.82%±2.56%vs 29.46%±1.83%,P<0.05);siRNA T2E以及KYA1797K实验组的DU145细胞Wnt 3α、β-catenin蛋白水平下调(P<0.05)。[结论]抑制TE融合基因表达后,DU145细胞的增殖速度与侵袭数量降低,凋亡率增加,该机制与TE融合基因调节Wnt/β-catenin途径相关。[Objective]To investigate the effect of TE fusion gene on the metastasis of prostate cancer DU145 cells.[Method]DU145 cells were randomly divided into three experimental groups:siRNA NC group,siRNA T2E group and KYA1797K group.The proliferation of DU145 cells was detected by MTT assay.Transwell assay was used to detect the invasion ability of DU145 cells.The apoptosis rate of DU145 cells was detected by flow cytometry.The protein expression of Wnt/β-catenin pathway in DU145 cells was analyzed by Western Blot.[Result]Compared with the siRNA NC group,the proliferation ability of DU145 cells in the siRNA T2E and KYA1797K groups decreased(1.73±0.35 us 1.02±0.22 vs 0.91±0.27,P<0.05).The invasion number of DU145 cells in siRNA T2E and KYA1797K groups decreased(167.28±12.89 us 78.21±9.26 us 82.33±10.33,P<0.05).The apoptosis rate of DU145 cells in siRNA T2E and KYA1797K groups was significantly increased(8.25%±0.89%us 28.82%±2.56%us 29.46%±1.83%,P<0.05).The protein expressions of Wnt3αandβ-catenin in DU145 cells in siRNA T2E group and KYA1797K group were down-regulated(P<0.05).[Conclusion]After inhibiting the expression of TE fusion gene,the proliferation rate and invasion number of DU145 cells was decreased,and the apoptosis rate is increased,which is related to the regulation of Wnt/β-catenin pathway by TE fusion gene.

关 键 词：TE融合基因 WNT 转移 β-catenin 前列腺癌 凋亡 DU145 siRNA 

分 类 号：R737.2[医药卫生—肿瘤]