流式细胞术在非造血系统肿瘤诊断和治疗监测中的临床应用进展  

Clinical application progress of flow cytometry in diagnosis and therapeutic monitoring of patients with nonhematopoietic neoplasms

作　　者：胡倩雯 杨肃文[1] 乔赛 谢鑫友 张钧[1] Hu Qianwen;Yang Suwen;Qiao Sai;Xie Xinyou;Zhang Jun(Department of Clinical Laboratory,Sir Run Run Shaw Hospital of Zhejiang University School of Medicine,Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province,Hangzhou 310000,China)

机构地区：[1]浙江大学医学院附属邵逸夫医院检验科、浙江省医学精准检验与监测研究重点实验室,杭州310000

出　　处：《中华检验医学杂志》2024年第7期722-728,共7页Chinese Journal of Laboratory Medicine

摘　　要：流式细胞术(FCM)是一项集光学、应用流体学、电子学和计算机等多门学科为一体的细胞分析技术。目前,FCM在血液系统肿瘤的诊断与治疗监测中广泛应用,但在非造血系统肿瘤(NHN)的临床应用仍处于初级阶段。近年来,随着分析需求的日益增长和科学技术的发展,一系列与现有高新技术高度整合的新型FCM逐步受到关注,例如质谱流式细胞术(CyTOF)、全光谱流式细胞术(SFC),这些新型FCM的出现为NHN患者临床实验室辅助诊断提供了更多的可能。该文阐述传统FCM、CyTOF与SFC原理及其在NHN诊断和治疗中的应用及前景,为FCM在临床检验的进一步拓展应用提供参考。Flow cytometry(FCM)is an interdisciplinary cell analysis technology that integrates optics,fluid dynamics,electronics,and computer science.While FCM is widely utilized in diagnosing and monitoring hematologic malignancies,its application in nonhematopoietic neoplasms(NHN)remains in its nascent stages.However,recent advancements in science and technology have led to the emergence of innovative FCM technologies,such as mass spectrometry flow cytometry(CyTOF)and spectral flow cytometry(SFC),offering promising avenues for their clinical application aiming to assist the clinical diagnosis of NHN patients.This review summarizes the features of fundamentals of traditional FCM,CyTOF,and SFC technologies,along with their applications and future prospective in NHN diagnosis and treatment,aiming to offer updated insights for the continued expansion and utilization of FCM technology in clinical laboratory settings.

关 键 词：流式细胞术 临床实验室技术 质谱流式细胞术 全光谱流式细胞术 肿瘤 

分 类 号：R730.4[医药卫生—肿瘤] R730.5[医药卫生—临床医学]