Plastid engineering with an efficient RNAi delivery system based on bacteriophage MS2 virus-like particles enhances plant resistance to cotton bollworm  

作　　者：Chunmei Jiang Jinqiu Fu Fujun Li Kai Xia Shengchun Li Ling Chang Ralph Bock Jiang Zhang 

机构地区：[1]State Key Laboratory of Biocatalysis and Enzyme Engineering,School of Life Sciences,Hubei Hongshan Laboratory,Hubei University,Wuhan 430062,China [2]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen518000,China [3]Max-Planck-Institut fur Molekulare Pflanzenphysiologie,Am Muhlenberg 1,14476Potsdam-Golm,Germany

出　　处：《Molecular Plant》2024年第7期987-989,共3页分子植物（英文版）

基　　金：the National Natural Science Foundation of China(32272634 and 32271912);the National Key R&D Program of China(2023YFC2607000);the Key R&D Program of Ningxia(2023BCF01045);the Key Program in AGIS under grant no.AGISZDXM-202304.

摘　　要：Dear Editor,RNA-based pesticides are approaching the market,offering great potential to control agricultural pests such as the coleopteran insect Diabrotica virgifera virgifera(Head et al.,2017).However,application to lepidopteran pests has been hampered by their inconsistent response to RNA interference(RNAi).As a notorious lepidoptera pest,Helicoverpa armigera feeds on over 300 species of host plants(Fu et al.,2022).Although transgenicplants expressingdouble-stranded RNAs or microRNAs that target essential insect genes can inhibit the growth of larvae,complete protection of the plants and efficient killing of this insect have not been achieved(Bally et al.,2020;Chung et al.,2021;Li et al.,2023).The relatively low RNAi efficiency in lepidopterans is mainly attributable to the degradation and intracellular sequestration of double-stranded RNA(Shukla et al.,2016;Zhu and Palli,2020).To overcome these obstacles and improve RNAi efficiency in H.armigera,we attempted to deliver RNAi molecules using a bacteriophage MS2 virus-like particle(VLP)-based delivery system(Wang et al.,2016).

关 键 词：OVERCOME offering APPROACHING 

分 类 号：Q94[生物学—植物学]