刺芫荽总黄酮提取工艺优化及其抑菌活性研究  

Study on Extraction Technology and Antibacterial Activity of Total Flavonoids from Eryngium foetidum L.

作　　者：吕双双 刘佳月 廉伟伟 刘金海 周敬 LV Shuangshuang;LIU Jiayue;LIAN Weiwei;LIU Jinhai;ZHOU Jing(College of Pharmacy and Chemical Engineering,Zhengzhou University of Industrial Technology,Zhengzhou 451150,China)

机构地区：[1]郑州工业应用技术学院药学与化学工程学院,河南郑州451150

出　　处：《生物化工》2024年第3期45-48,共4页Biological Chemical Engineering

摘　　要：目的:刺芫荽总黄酮提取工艺优化并研究抑菌作用。方法:采用超声提取方法提取刺芫荽中的总黄酮,基于单因素实验分析并利用正交实验来优化刺芫荽中的总黄酮提取工艺,通过滤纸片法和肉汤稀释法实验测出最小抑菌浓度(MIC),进而研究刺芫荽黄酮类成分对大肠杆菌、金黄色葡萄球菌的抑制能力。结果:最佳提取工艺为超声温度70℃、超声时间40 min、乙醇体积分数80%、料液比1∶25(g∶mL),此条件下总黄酮提取率为1.480%。刺芫荽总黄酮对金黄色葡萄球菌的抑制作用大于大肠杆菌,最小抑菌浓度分别为0.250 mg/mL和0.500 mg/mL。结论:优化后的超声提取工艺方法可行性强,稳定性好,操作简单,为刺芫荽总黄酮的进一步开发奠定了基础。Objective:To optimize the extraction process of total flavonoids from Eryngium foetidum L.and study their antibacterial effects.Methods:Ultrasound extraction is used to extract total flavonoids from Eryngium foetidum L.Based on single factor experimental analysis and orthogonal experiments,the extraction process of total flavonoids from Eryngium foetidum L.is optimized.The minimum inhibitory concentration(MIC)is measured by filter paper method and broth dilution method,and the inhibitory ability of Eryngium foetidum L.flavonoids on Escherichia coli and Staphylococcus aureus is studied.Results:The optimal extraction process is ultrasound temperature of 70℃,ultrasound time of 40 minutes,ethanol volume fraction of 80%,and solid-liquid ratio of 1 to 25(g to mL).Under these conditions,the total flavonoid extraction rate is 1.480%.The total flavonoids of Eryngium foetidum L.have a greater inhibitory effect on Staphylococcus aureus than Escherichia coli,with minimum inhibitory concentrations of 0.250 mg/mL and 0.500 mg/mL,respectively.Conclusion:The optimized ultrasonic extraction process has strong feasibility,good stability,and simple operation,laying a foundation for the further development of total flavonoids in Eryngium foetidum L..

关 键 词：刺芫荽 总黄酮 提取工艺优化 抑菌活性 

分 类 号：Q946.8[生物学—植物学]