Enhancing the peroxidase-like activity of MoS_(2)-based nanozymes by introducing attapulgite for antibacterial application and sensitive detection of glutathione  

作　　者：Feng Feng Yihe Zhang Xiao Zhang Bin Mu Jiahe Zhang Wenjie Qu Wangshu Tong Minmin Liang Qi An Zhanjun Guo Lu Zhao 

机构地区：[1]Engineering Research Center of Ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources,Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes,National Laboratory of Mineral Materials,School of Materials Science and Technology,China University of Geosciences(Beijing),Beijing 100083,China [2]Key Laboratory of Clay Mineral Applied Research of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China [3]Experimental Center of Advanced Materials School of Materials Science and Engineering,School of Materials Science and Engineering,Beijing Institute of Technology,Beijing 100081,China

出　　处：《Nano Research》2024年第8期7415-7426,共12页纳米研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.52072347 and 22272152);the Foundation of Key Laboratory of Clay Mineral Applied Research of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences(No.CMAR-2022-04);Beijing Nova project(No.20220484155).

摘　　要：Nanozymes are next-generation of nanomaterials with enzyme-like activities.In particular,nanozymes with peroxidase(POD)-like activity have been utilized in various fields,including antibacterial,detection,degradation,etc.However,their extensive applications were limited by their low catalytic activity currently.Herein,we have presented a composite nanozyme based on attapulgite(ATP)(Fe-ATP-MoS_(2)(FAM)),which exhibited enhanced POD-like activity(185.33 U·mg^(−1)),4.25 times higher than that of Fe-MoS_(2)(FM)(43.63 U·mg^(−1)).The density functional theory(DFT)calculations indicated that the addition of ATP increased the electron density of metal centers(Mo and Fe).More importantly,Michaelis–Menten kinetics revealed that the introduction of ATP significantly enhanced the binding affinities of substrates through the pores of ATP,forming a highly concentrated substrate microenvironment and thus promoting its POD-like activity.Additionally,from molecular size and kinetic analysis,we proposed that the changes in substrate size before and after oxidation also significantly affected its Michaelis-constant(Km)value.Furthermore,we utilized FAM in the applications of highly effective antibacterial application and sensitive detection of glutathione(GSH).In conclusion,this work provides a novel approach for designing a highly efficient nanozyme based on natural mineral composites.

关 键 词：ATTAPULGITE PEROXIDASE electron density AFFINITY ANTIBACTERIAL 

分 类 号：O64[理学—物理化学]