Enhancing osteogenic bioactivities of coaxial electrospinning nanoscaffolds through incorporating iron oxide nanoparticles and icaritin for bone regeneration  

作　　者：Peng Wang Qianjin Wang Dengxian Wu Yunyang Zhang Shixiong Kang Xucai Wang Jiayu Gu Hao Wu Zhihong Xu Qing Jiang 

机构地区：[1]School of Biomedical Engineering and Informatics,Nanjing Medical University,Nanjing 211166,China [2]State Key Laboratory of Pharmaceutical Biotechnology,Department of Sports Medicine and Adult Reconstructive Surgery,Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,Nanjing 210008,China [3]Center of Modem Analysis,Nanjing University,Nanjing 210008,China [4]State Key Laboratory of Bioelectronics,Jiangsu Key Laboratory for Biomaterials and Devices,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210009,China [5]Co-Innovation Center for Efficient Processing and Utilization of Forest Resources,College of Chemical Engineering,Nanjing Forestry University,Nanjing 210037,China [6]Jiangsu Institute of Metrology,Nanjing 210023,China

出　　处：《Nano Research》2024年第7期6430-6442,共13页纳米研究（英文版）

基　　金：supported by the National Basic Research Program of China(Nos.2021YFA1201404,and 2019YFA0210103);the National Natural Science Foundation of China(Nos.32271413,and 82272492);Natural Science Foundation of Jiangsu Province(No.BK20232023);Science program of Jiangsu Province Administration for Market Regulation(No.KJ2024010).

摘　　要：Bone tissue engineering provides a promising strategy for the treatment of bone defects.Nonetheless,the clinical utilization of biomaterial-based scaffolds is constrained by their inadequate mechanical strength and absence of osteo-inductive properties.Here,we proposed to endow nano-scaffold(NS)constructed by coaxial electrospinning technique with enhanced osteogenic bioactivities and mechanical properties by incorporating biocompatible magnetic iron oxide nanoparticles(IONPs)and icaritin(ICA).Four types of nano-scaffolds(NS,ICA@NS,NS-IONPs and ICA@NS-IONPs)were prepared.The incorporation of ICA and IONPs minimally impact their surface morphological and chemical properties.IONPs enhanced the mechanical properties of NS scaffolds,including hardness,tensile strength,and elastic modulus.In vitro assessments demonstrated that ICA@NS-IONPs exhibited enhanced osteogenic bioactivities towards mouse calvarial pre-osteoblast cell line MC3T3-E1 as evidenced by detecting the alkaline phosphatase(ALP)activity level,expressions of osteogenesis-related genes and proteins as well as mineralized nodule formation.Mechanistic investigations revealed that MEK/ERK(MAP kinase-ERK kinase(MEK)/extracellularsignal-regulated kinase(ERK))signaling pathway could offer a plausible explanation for the osteogenic differentiation of MC3T3-E1 cells induced by [e-mail],the implantation of nano-scaffolds in rat skull defects exhibited a substantial improvement in in vivo bone regeneration.Therefore,IONPs and ICA incorporated coaxial electrospinning nano-scaffolds present a novel strategy for the optimization of scaffolds for bone tissue engineering.

关 键 词：iron oxide nanoparticles ICARITIN coaxial electrospinning nano-scaffolds MEK/ERK signaling pathway bone regeneration 

分 类 号：R318[医药卫生—生物医学工程]