片仔癀对情志内热诱导流感病毒易感性的作用研究  

Effect of Pien Tze Huang on emotional stress-induced influenza virus susceptibility

作　　者：王容 陈新星 黄睿婷 孙万阳[2,3,4,5] 何蓉蓉[2,3,4,5] 李怡芳[2,3,4,5,6] 黄丰 WANG Rong;CHEN Xin-xing;HUANG Rui-ting;SUN Wan-yang;HE Rong-rong;LI Yi-fang;HUANG Feng(School of Chinese Materia Medica,Yunnan University of Chinese Medicine,Kunming 650500,China;Guangdong Engineering Research Center of Chinese Medicine and Disease Susceptibility,Jinan University,Guangzhou 510632,China;Guangdong Engineering Research Center of Traditional Chinese Medicine&Health Products,Jinan University,Guangzhou 510632,China;Institute of Traditional Chinese Medicine and Natural Products,Jinan University,Jinan University,Guangzhou 510632,China;Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research,Jinan University,Guangzhou 510632,China;Jiangmen Traditional Chinese Medicine Hospital Affiliated to Jinan University,Jiangmen,Guangdong 529099,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]暨南大学广东省疾病易感性及中医药研发工程技术研究中心,广东广州510632 [3]暨南大学中医药与大健康广东省工程研究中心,广东广州510632 [4]暨南大学中药及天然药物研究所,广东广州510632 [5]暨南大学广东省中药药效物质基础及创新药物研究重点实验室,广东广州510632 [6]暨南大学附属江门中医院,中国江门529099

出　　处：《中国药理学通报》2024年第8期1565-1572,共8页Chinese Pharmacological Bulletin

基　　金：广东省基础与应用基础研究基金(No.2023B1515040016);广东省粤港澳高校联合实验室(No.2023LSYS002);广州市疾病易感性与中医药防治重点实验室(No.2024A03J090)。

摘　　要：目的本研究在中医情志致病理论指导下,基于课题组前期利用小鼠拘束应激附加H1N1感染构建情志内热诱导流感病毒易感模型,评价了名优清热中成药片仔癀对流感病毒易感性的作用。方法小鼠拘束应激18 h后滴鼻感染H1N1流感病毒,记录小鼠的体征和体质量变化,统计各组小鼠的发病率。在病毒感染后的第4日取肺脏组织采用HE染色、免疫组化、Western blot、ELISA等方法检测病理情况和炎症水平;分别通过Western blot和试剂盒检测脂质过氧化终产物水平;利用氧化磷脂组学检测肺组织中各类氧化磷脂的含量。结果片仔癀能够显著降低情志应激小鼠感染流感病毒的发病率,抑制肺组织流感病毒复制与炎症因子释放,改善肺部炎症,缓解脂质过氧化终产物堆积。氧化磷脂组学结果显示,情志应激附加流感感染小鼠肺组织中氧化磷脂酰胆碱和氧化磷脂酰乙醇胺水平均明显增加,片仔癀给药能显著降低磷脂过氧化水平。结论清热中药片仔癀对情志内热诱导的流感病毒易感有良好的改善作用,作用机制可能与调节磷脂过氧化有关。Aim To evaluate the effect of Pien Tze Huang(PTH)on influenza virus susceptibility in restraint stress-induced H1N1 influenza susceptibility model in mice with emotional disorders and internal heat,guided by the theory of emotional pathogenesis.Methods Mice were infected with H1N1 influenza virus following 18 h of restraint stress.The signs and weight changes of mice were recorded,and the morbidity of mice were analyzed.On the fourth day post viral infection,the lung tissue was collected.The pathological changes and inflammatory factors in lungs were detected by HE staining.The expression of NP was assessed by immunohistochemistry and Western blot.The level of lipid peroxidation end products was detected using a commercial kit and western blot.Redox phospholipidomics was analyzed in lung tissue by HPLC-MS/MS.Results PTH significantly reduced the mortality of influenza-susceptible mice induced by emotional stress,inhibited the expression of NP and the release of inflammatory factors,improved inflammation in lung tissue,and alleviated the accumulation of lipid peroxidation end products.Phospholipid oxidation analysis revealed the elevated levels of oxidized phospholipid choline and phosphatidylethanolamine in lung tissue of influenza-susceptible mice,which were significantly reduced by PTH administration.Conclusions PTH exhibits promising efficacy in ameliorating influenza virus susceptibility induced by internal heat,and its mechanism of action may be related to the regulation of phospholipid peroxidation.

关 键 词：情志应激 片仔癀 清热中药 病毒性肺炎 氧化应激 脂质过氧化 

分 类 号：R-332[医药卫生] R282.71R282.74R349.1R344.5R563.12