8%盐酸脱钙液处理骨髓组织时长与染色效果的探索  

Timing and staining effect of 8%hydrochloric acid decalcification solution on bone marrow tissue

作　　者：孙改霞 钟定荣 陈煌 刘博[1] 刘军超[1] 张凡[1] SUN Gai-xia;ZHONG Ding-rong;CHEN Huang;LIU Bo;LIU Jun-chao;ZHANG Fan(Department of Pathology,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China;Department of Pathology,China-Japan Friendship Hospital,Bejing 100021,China)

机构地区：[1]河北北方学院附属第一医院病理科,河北张家口075000 [2]中日友好医院病理科,北京100021

出　　处：《诊断病理学杂志》2024年第6期494-497,531,共5页Chinese Journal of Diagnostic Pathology

基　　金：河北省卫生厅科研课题(编号:20170776)。

摘　　要：目的探索8%盐酸脱钙液处理骨髓组织不同时长的染色效果。方法收集骨髓组织活检标本180例,利用8%盐酸脱钙液分别在70 min,90 min,110 min处理骨髓组织,观察不同脱钙处理时长的骨髓组织HE染色、免疫组织化学染色(EnVision法染TdT、CD56、MPO)和特殊染色(Gomori网状纤维染色)效果。结果8%盐酸脱钙液处理90 min与70 min和110 min相比,骨髓组织石蜡切片组织结构保持较完整,HE染色后形态结构清晰可辨,对比度较好,免疫组化染色定位准确、背景清楚,网状纤维染色粗细清晰可辨。结论8%盐酸脱钙液对骨髓组织90 min脱钙效果较好。Objective To explore the staining effect of 8%hydrochloric acid decalcification solution on bone marrow tissue with different duration.Methods A total of 180 cases of bone marrow biopsy samples were collected,and the bone marrow tissues were treated with 8%hydrochloric acid decalcifier at 70 min,90 min and 110 min,respectively.The effects of HE staining,immunohistochemical staining(EnVision method staining TdT,CD56,MPO)and special staining(Gomori reticular fiber staining)on bone marrow tissues of different decalcification treatment periods were observed.Results Compared with 70 min and 110 min treated with 8%hydrochloric acid decalcification solution,the tissue structure of paraffin sections of bone marrow tissue remained more complete.After HE staining,the morphological structure was clear and distinguishable,the contrast was good,the immunohistochemical staining was accurate and the background was clear,and the staining thickness of mesh fibers was clearly distinguishable.Conclusion 8%hydrochloric acid decalcification solution has good effect on bone marrow tissue decalcification in 90 min.

关 键 词：骨髓组织 8%盐酸脱钙液 HE染色 网状纤维染色 免疫组化染色 

分 类 号：R733.3[医药卫生—肿瘤]