补肾调肝降浊方对肥胖多囊卵巢综合征大鼠白色脂肪褐变的影响  

Effects of Bushen Tiaogan Jiangzhuo Formula on white fat browning in obese polycystic ovary syndrome rats

作　　者：庞颖 刘雁峰[2] 吴豆豆 孔鑫靓 莫婷婷[3] 陈然 PANG Ying;LIU Yanfeng;WU Doudou;KONG Xinliang;MO Tingting;CHEN Ran(Beijing Nuclear Industry Hospital,Beijing 100045,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京核工业医院,北京100045 [2]北京中医药大学东直门医院,北京100700 [3]北京中医药大学,北京100029

出　　处：《中华中医药杂志》2024年第7期3729-3734,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：首都卫生发展科研专项项目(No.2020-2-4194)。

摘　　要：目的:探讨补肾调肝降浊方治疗肥胖型多囊卵巢综合征(PCOS)的可能作用机制。方法:将60只6周龄SPF级SD雌性大鼠分为空白组(10只)和造模组(50只),造模组采用来曲唑灌胃联合高脂饮食喂养制备肥胖型PCOS大鼠模型,将造模成功的50只大鼠随机分为模型组,中药低、中、高剂量组和二甲双胍组。给药结束后,ELISA法检测血清中瘦素(LP)、脂联素(ADPN);Western Blot及RT-qPCR法检测大鼠白色脂肪组织(WAT)和棕色脂肪组织(BAT)中腺苷酸活化蛋白激酶(AMPK)、过氧化物酶增殖激活受体-γ共激活子-1α(PGC-1α)、解偶联蛋白1(UCP-1)蛋白及mRNA表达情况。结果:与空白组比较,模型组大鼠LP显著升高和ADPN显著降低(P<0.01);与模型组比较,中药低、中、高剂量组LP显著降低(P<0.01),中药中、高剂量组ADPN显著升高(P<0.05)。与模型组比较,中药中剂量组可以激活WAT中AMPK/PGC-1α通路及提高UCP-1的表达(P<0.01);二甲双胍组亦可激活WAT中AMPK/PGC-1α通路(P<0.05,P<0.01)。与空白组比较,模型组BAT中AMPK、PGC-1α、UCP-1 mRNA和蛋白表达降低(P<0.05,P<0.01);与模型组比较,中药中剂量组AMPK、PGC-1α、UCP-1 mRNA和蛋白表达显著升高(P<0.01,P<0.05)。结论:补肾调肝降浊方可以通过激活肥胖型PCOS模型大鼠AMPK/PGC-1α通路,提高UCP-1表达,降低LP,提高ADPN水平,促使WAT褐变,激活BAT,使机体的脂代谢恢复正常,降低游离睾酮,恢复排卵。Objective:To explore the possible mechanism of Bushen Tiaogan Jiangzhuo Formula in the treatment of obese polycystic ovary syndrome(PCOS).Method:Sixty 6-week-old SPF grade SD female rats were divided into blank group(10 rats)and marking model group(50 rats).The making model group was fed with letrozole by gavage and a high-fat diet to prepare an obese PCOS rat model.The 50 rats that successfully established the model were randomly divided into model group,low,medium and high dose group of Bushen Tiaogan Jiangzhuo Formula,and metformin group.After the administration,the ELISA method was used to detect serum leptin(LP)and adiponectin(ADPN);Western Blot and RT-qPCR were used to detect the protein and mRNA expression of AMPK,PGC-1α,and UCP-1 in white adipose tissue(WAT)and brown adipose tissue(BAT)of rats.Results:Compared with the blank group,LP increased in the model group and ADPN decreased(P<0.01);the LP of the low,medium,and high dose group of Bushen Tiaogan Jiangzhuo Formula was significantly lower than that of the model group(P<0.01),while the ADPN of the medium and high dose groups of Bushen Tiaogan Jiangzhuo Formula was significantly increased(P<0.05).Compared with the model group,the medium dose group of Bushen Tiaogan Jiangzhuo Formula could activate the WAT AMPK/PGC-1αpathway and increase the expression of UCP-1(P<0.01);Metformin also activated the AMPK/PGC-1αpathway(P<0.05,P<0.01).Compared with the control group,detection of BAT revealed a decrease in the mRNA and protein expression of AMPK,PGC-1α,and UCP-1 in the model group(P<0.05,P<0.01);the mRNA and protein expression of AMPK,PGC-1αand UCP-1 in the medium dose group of Bushen Tiaogan Jinagzhuo Formula significantly increased(P<0.01,P<0.05).Conclusion:Bushen Tiaogan Jiangzhuo Formula can activate the AMPK/PGC-1αpathway,increase the expression of UCP-1,reduce LP,increase ADPN levels,promote WAT browning,activate BAT,restore normal lipid metabolism,reduce FT,and restore ovulation.

关 键 词：补肾调肝降浊方 多囊卵巢综合征 肥胖 白色脂肪组织 褐变 棕色脂肪组织 机制 

分 类 号：R285.5[医药卫生—中药学]