免疫组织化学特异性抗体诊断浆膜腔积液转移性腺癌与增生性间皮细胞的应用价值  

Application Value of Immunohistochemistry Specific Antibodies in the Diagnosis of Metastatic Adenocarcinoma And Proliferative Mesothelial Cells in Effusion in the Serous Cavity

作　　者：赵维聪 彭江兰 牙冬雪 陈盈 冯敏 韦丽洙 Zhao Weicong;Peng Jianglan;Ya Dongxue;Chen Ying;Feng Min;Wei Lizhu(Guangxi Huayin Medical Laboratory,Nanning Guangxi 530031,China;Laboratory Department NO.9 People's Hospital Of Nanning,Nanning Guangxi 530409,China)

机构地区：[1]广西华银医学检验所,广西南宁530031 [2]南宁市第九人民医院检验科,广西南宁530409

出　　处：《生命科学仪器》2024年第2期64-65,69,共3页Life Science Instruments

基　　金：南宁市科技局重点研发计划项目(20193123)。

摘　　要：目的探讨免疫组织化学特异性抗体诊断浆膜腔积液转移性腺癌(MA)与增生性间皮细胞的应用价值。方法选取2021年1月至2023年10月广西华银医学检验所病理科浆膜腔积液标本181例,其中,113例有转移性腺癌细胞,68例有增生性间皮细胞。制备细胞石蜡切片,采用免疫组织化学标记法检测抗体绒毛蛋白(Villin)、细胞角蛋白7(CK 7)、上皮特异抗原(Ber-Ep 4)、上皮细胞表面糖蛋白单克隆抗体(MOC-31)、癌胚抗原(CEA)水平,分析上述抗体诊断MA与增生性间皮细胞的应用价值。结果Villin、CK7、Ber-Ep4、MOC-31及CEA鉴别诊断转移性腺癌细胞与增生性间皮细胞的灵敏度分别为54.87%、69.91%、78.76%、93.81%、81.42%,特异度分别为94.12%、92.65%、88.24%、92.65%、92.65%。结论免疫组织化学特异性抗体鉴别诊断浆膜腔积液中转移性腺癌细胞与增生性间皮细胞的准确性高,在细胞病理学诊断中的应用价值较高。Objective:To explore the application value of immunohistochemical specific antibody in the diagnosis of metastatic adenocarcinoma(MA)and proliferative mesothelial cells in serous cavity effusion.Methods:From January 2021 to October 2023,181 cases of serous cavity effusion in pathology department of Guangxi Huayin Medical Inspection Institute were selected,among which 113 cases had metastatic gonadal cancer cells and 68 cases had proliferative mesothelial cells.Paraffin sections of cells were prepared,and the levels of antibody Villin,cytokeratin 7(CK 7),epithelial specific antigen(Ber-Ep 4),monoclonal antibody MOC-31 against glycoprotein on epithelial cell surface and carcinoembryonic antigen(CEA)were detected by immunohistochemical method,and the application value of these antibodies in diagnosing MA and proliferative mesothelial cells was analyzed.Results:The sensitivity of Villin、CK7、Ber-Ep4、MOC-31 and CEA in differential diagnosis of metastatic gonadal cancer cells and proliferative mesothelial cells were 54.87%、69.91%、78.76%、93.81%and 81.42%,and the specificity was 94.12%、92.65%and 88.24%,respectively.Conclusion:Immunohistochemical specific antibody has high accuracy in differential diagnosis of metastatic gonadal cancer cells and proliferative mesothelial cells in serous cavity effusion,and has high application value in cytopathological diagnosis.

关 键 词：浆膜腔积液 免疫组织化学 抗体 转移性腺癌细胞 增生性间皮细胞 

分 类 号：R730.4[医药卫生—肿瘤]