糙皮侧耳多功能过氧化物酶基因异源表达及其对玉米秸秆木质素的降解  

Expression of a Pleurotus ostreatus Versatile Peroxidase Gene in Pichia pastoris and Its Degradation of Corn Stover Lignin

作　　者：宋倩 邓勋[2] 宋瑞清[1] SONG Qian;DENG Xun;SONG Ruiqing(College of Forestry,Northeast Forestry University,Harbin 150040,China;Institute of Forest Protection,Heilongjiang Academy of Forestry,Harbin 150040,China)

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]黑龙江省林业科学院森林保护研究所,哈尔滨150040

出　　处：《吉林农业大学学报》2024年第3期399-406,共8页Journal of Jilin Agricultural University

基　　金：国家自然科学基金面上项目(31470648)。

摘　　要：将糙皮侧耳多功能过氧化物酶(Versatile peroxidase)基因转入到毕赤酵母菌株X33中并实现表达,测定重组酶的酶学性质,并测试其对玉米秸秆木质素的降解能力。结果表明:重组多功能过氧化物酶对2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸、2,6-二甲氧基苯酚、2-甲氧基酚和藜芦醇进行分解的最适pH分别为2.5,3,3.5和4.5,除分解藜芦醇的最适反应温度为40℃外,其他底物的分解最适温度均为50℃,且具有良好的热稳定性及耐酸碱性。重组多功能过氧化物酶和原始糙皮侧耳对玉米秸秆中木质素的降解率分别为23.59%,10.93%。研究结果为多功能过氧化物酶对木质素降解机理提供了理论依据。The Pleurotus ostreatus versatile peroxidase gene was transferred into Pichia pastoris strain X33 and expression was achieved.The enzymatic properties of recombinant yeast were determined and its ability to degrade corn stover lignin was determined.The results showed that the optimal pH values for the decomposition of 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid,2,6-dimethoxyphenol,2-methoxyphenol and veratryl alcohol by recombinant versatile peroxidase were 2.5,3,3.5 and 4.5,respectively.Except for the optimum reaction temperature for decomposition veratryl alcohol,which was 40 C,the optimum temperature of other substrates was 50 C,and it had good thermal stability and acid and alkali resistance.The degradation rates of lignin in corn stover by recombinant multifunctional peroxidase and by native Pleurotus ostreatus were 23.59%and 10.93%,respectively.The results provide a theoretical basis for studying the mechanism of lignin degradation by versatile peroxidase.

关 键 词：糙皮侧耳 多功能过氧化物酶 毕赤酵母 木质素 降解能力 

分 类 号：S646.14[农业科学—蔬菜学]