基于人参多功能微生物菌肥创制的食用菌菌糠筛选  

Screening of Spent Mushroom Substrates Based on the Development of Multifunctional Microbial Fertilizer on Ginseng

作　　者：邵郅伟 杨一帆 张海翔 石忠锋 卢宝慧 王雪 高洁 SHAO Zhiwei;YANG Yifan;ZHANG Haixiang;SHI Zhongfeng;LU Baohui;WANG Xue;GAO Jie(College of Plant Protection,Jilin Agricultural University,Changchun 130118,China;Jiarunhe Mushroom Planting Cooperative,Panjin 124219,China)

机构地区：[1]吉林农业大学植物保护学院,长春130118 [2]盘锦嘉润禾食用菌种植合作社,盘锦124219

出　　处：《吉林农业大学学报》2024年第3期407-414,共8页Journal of Jilin Agricultural University

基　　金：吉林省科技发展计划项目(20200402072NC)。

摘　　要：为了筛选出用于多功能微生物菌肥创制的食用菌菌糠及活性菌株,采用平板对峙法评价了11种食用菌菌糠浸提液对人参8种主要病原菌的抑菌活性,同时采用抑菌圈法测定了菌糠浸提液与对人参病菌具有明显生防活性的6株生防细菌之间的作用。结果表明:猴头菇和杏鲍菇2种食用菌菌糠浸提液对人参8种主要病原菌具有较为广谱的抑菌活性,其中对人参立枯病菌和灰葡萄孢病菌的抑菌效果最明显,抑菌圈直径最高,分别为29.33 mm和30.33 mm,对FS6(Bacillus amyloliquefaciens)等6株生防细菌均未表现抑制作用。该研究为人参多功能生物菌肥的创制奠定了基础,为食用菌菌糠绿色回收循环利用提供新途径。In order to screen out spent mushroom substrates(SMS)and active strains for the formulation of multi-functional microbial fertilizer,the inhibitory activity of 11 kinds of SMS extracts against 8 main pathogens on ginseng was evaluated by plate confrontation method,and the effects of SMS on 6 biocontrol bacteria were evaluated by inhibitory zone method.The results showed that the extracts of SMS from Hericium erinaceus and Pleurotus eryngii had broad-spectrum antimicrobial activities against 8 major pathogenic fungi on Panax ginseng,and the obvious inhibitory effect on Rhizoctonia solani and Botrytis cinerea,the maximum diameters of inhibition zone reached 29.33 mm and 30.33 mm,respectively and they had no antagonistic activity against 6 biocontrol bacteria such as Bacillus amyloliquefaciens.This study will lay a foundation for the creation of ginseng multifunctional microbial fertilizer,and it provides a new way for green recycling of spent mushroom substrates.

关 键 词：人参 微生物菌肥 食用菌菌糠 抑菌活性 生防菌株 

分 类 号：S646[农业科学—蔬菜学]