大肠进展期腺瘤舌苔菌群微生态分析  

Microecology of tongue flora in patients with advanced colorectal adenoma

作　　者：黄朔 王林恒[2] 江贵 丁玄 张丽杰 HUANG Shuo;WANG Linheng;JIANG Gui;DING Xuan;ZHANG Lijie(Beijing University of Chinese Medicine,Beijing 100029;Department of Spleen,Stomach,Liver and Gallbladder,)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院脾胃肝胆科

出　　处：《胃肠病学和肝病学杂志》2024年第7期837-843,共7页Chinese Journal of Gastroenterology and Hepatology

基　　金：北京中医药大学重点攻关项目(2020-JYB-ZDGG-132)。

摘　　要：目的探究大肠进展期腺瘤患者舌苔菌群微生态的特点。方法纳入2023年2月至2023年4月就诊于北京中医药大学东方医院消化内镜中心的大肠进展期腺瘤患者共15例,体检中心健康人共30名,使用口腔咽拭子刮取舌苔,进行16S rRNA扩增并进行高通量测序,对测序结果进行微生态分析。结果大肠进展期腺瘤组与健康人群组在菌群的Alpha多样性方面差异无统计学意义(P>0.05);Beta多样性方面差异有统计学意义(P<0.05)。大肠进展期腺瘤组中放线菌门(Actinobacteria)、奇异菌属(Atopobium)、放线菌属(Actinomyces)、罗氏菌属(Rothia)的相对丰度显著高于健康人群组(P<0.05)。大肠进展期腺瘤组中巨球形菌属(Megasphaera)、Oribacterium、韦荣氏球菌属(Veillonella)相对丰度显著低于健康人群组(P<0.05)。结论大肠进展期腺瘤患者的舌苔菌群与健康人群相比存在差异,其中主要表现在奇异菌属(Atopobium)、放线菌属(Actinomyces)、罗氏菌属(Rothia)的增加,巨球形菌属(Megasphaera)、Oribacterium、韦荣氏球菌属(Veillonella)丰度的减少。Objective To investigate the characteristics of the microecology of tongue flora in patients with advanced colorectal adenoma.Methods A total of 15 patients with advanced colorectal adenoma who attended the Digestive En-doscopy Center of Dongfang Hospital,Beijing University of Chinese Medicine from Feb.2023 to Apr.2023,and 30 healthy people from the physical examination center were included.The tongue was scraped using oral pharyngeal swabs.16S rRNA amplification and the high-throughput sequencing were performed.Microecological analysis was con-ducted on the sequencing results.Results There was no significant difference in Alpha diversity between the advanced colorectal adenoma group and healthy people group(P>0.05).The difference in Beta diversity was statistically signifi-cant(P<0.05).The relative abundance of Actinobacteria,Atopobium,Actinomyces and Rothia in the advanced colorec-tal adenoma group was significantly higher than that of healthy people group(P<0.05).The relative abundance of Me-gasphaera,Oribacterium and Veillonella in advanced colorectal adenoma group was significantly lower than that in healthy people group(P<0.05).Conclusion The tongue flora of patients with advanced colorectal adenoma is different from that of healthy people,which is mainly manifested in the increased abundance of Atopobium,Actinomyces and Rothia.Decrease in the abundance of Megasphaera,Oribacterium and Veillonella.

关 键 词：大肠进展期腺瘤 舌苔 菌群 微生态 

分 类 号：R735.3[医药卫生—肿瘤] R241.25[医药卫生—临床医学]