墨旱莲黄酮类提取物对运动大鼠抗疲劳作用的研究  

Study on the Anti-Fatigue Effect of Flavonoids Extracts from Eclipta alba on Exercise Rats

作　　者：李源馨 LI Yuanxin(Huanghuai University,Zhumadian 463000,China)

机构地区：[1]黄淮学院,河南驻马店463000

出　　处：《食品安全导刊》2024年第14期67-69,共3页China Food Safety Magazine

基　　金：河南省科技攻关计划项目“抗坏血酸处理下发芽糙米、绿豆、玉米植酸降解及低级肌醇磷酸富集技术”(182102110126);河南省科技攻关计划项目“乳酸菌缓解黄曲霉毒素B1对鸡胚原代肝细胞炎症反应的分子机制”(222300420238)。

摘　　要：目的:研究大鼠药物干预后的力竭时间、血清生化指标和糖原变化,为墨旱莲黄酮类提取物应用于缓解运动性疲劳、提高运动能力等提供依据。方法:健康雄性大鼠40只,随机分成安静组(Q)、运动组(S)、墨旱莲安静组(EQ)和墨旱莲运动组(ES),每组10只,安静组和运动组正常喂养,墨旱莲安静组和墨旱莲运动组每天按800 mg·kg~(-1)的剂量服用一次,运动组大鼠在跑台上进行6周训练,第6周最后一天给药30 min后,4组大鼠进行强迫性坡度为0°的力竭跑台运动。力竭后麻醉采血,对血清指标、血糖、肌糖原和肝糖原进行测试。数据采用SPSS17.0作方差分析和均数比较。结果:墨旱莲黄酮类提取物能够延长大鼠的力竭时间,墨旱莲黄酮类提取物联合运动锻炼能够有效地抑制由于运动导致的血清指标升高,有效提高血糖、肌糖原和肝糖原水平。结论:墨旱莲黄酮类提取物配合运动可以有效地提高机体的抗自由基、抗氧化能力,提高肝糖原和肌糖原储备量和利用率,降低运动对肝脏和骨骼肌的损伤,提高运动能力。Objective:To study the changes in exhaustion time,serum biochemical indicators,and glycogen levels after drug intervention in rats,and to provide evidence for the use of flavonoid extracts from Eclipta alba in alleviating exercise induced fatigue and improving exercise ability.Method:Forty healthy male rats were randomly divided into a quiet group(Q),an exercise group(S),an Eclipta alba quiet group(EQ),and an Eclipta alba exercise group(ES),with 10 rats in each group.The quiet group and exercise group were fed normally,while the Eclipta alba quiet group and Eclipta alba exercise group were given a daily dose of 800 mg·kg-1.The exercise group rats were trained on a treadmill for 6 weeks,and after 30 minutes on the last day of the 6th week,the four groups of rats underwent forced exhaustion treadmill exercise with a slope of 0°.Blood was collected under anesthesia after exhaustion,and serum indicators,blood sugar,muscle glycogen,and liver glycogen were tested.The data was analyzed using SPSS 17.0 for analysis of variance and mean comparison.Results:Flavonoids extract compounds of Eclipta alba were able to prolong the exhaustion time of rats,and Eclipta alba flavonoids extract compounds combined with exercise were able to effectively inhibit the elevation of enzyme activities and metabolite serum indexes due to exercise,and effectively increase the levels of blood glucose,myo-glycogen,and hepatic glycogen.Conclusion:The combination of flavonoids extracts from Eclipta alba with exercise can effectively improve the body’s anti-free radicals and antioxidant capacity,increase the reserve and utilization of hepatic glycogen and myoglycogen,reduce the damage of exercise to liver and skeletal muscle,and improve the exercise capacity.

关 键 词：墨旱莲 黄酮类提取物 运动大鼠 抗疲劳 

分 类 号：R285.5[医药卫生—中药学]