血清MMP1和P53自身抗体联合检测对食管鳞状细胞癌的诊断价值  

Detection of both serum MMP1 and P53 autoantibody for the diagnosis of esophageal squamous cell carcinoma

作　　者：黄小燕[1] 伍方财[2] 柳灿烔 黄佳涛 许镒洧[3,4] HUANG Xiaoyan;WU Fangcai;LIU Cantong;HUANG Jiatao;XU Yiwei(Department of Anaesthesiology,the Cancer Hospital of Shantou University Medical College,Shantou 515041;Department of Radiation Oncology,the Cancer Hospital of Shantou University Medical College,Shantou 515041;Department of Clinical Laboratory Medicine,Esophageal Cancer Prevention and Control Research Center,the Cancer Hospital of Shantou University Medical College,Shantou 515041;Guangdong Esophageal Cancer Institute,Guangzhou 510060,Guangdong,China)

机构地区：[1]汕头大学医学院附属肿瘤医院麻醉科,广东汕头505141 [2]汕头大学医学院附属肿瘤医院放疗科,广东汕头505141 [3]汕头大学医学院附属肿瘤医院检验科,食管癌综合防治研究中心,广东汕头505141 [4]广东省食管癌研究所,广东广州510060

出　　处：《癌变．畸变．突变》2024年第4期305-308,324,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：汕头市科技计划项目(200605115266724)。

摘　　要：目的:探讨食管鳞状细胞癌(ESCC)患者血清基质金属蛋白酶1(MMP1)和P53自身抗体表达水平及其联合检测的诊断意义。方法:收集2013年3—9月在汕头大学医学院附属肿瘤医院住院治疗的93例ESCC患者,并以同期90例体检者为正常对照组。应用酶联免疫吸附实验(ELISA)检测两组对象血清MMP1和P53自身抗体的表达水平,采用受试者工作特征(ROC)曲线评价诊断效能。结果:血清MMP1含量和P53自身抗体滴度在ESCC组分别为(8.076±5.912)ng/mL和0.291±0.492,正常对照组分别为(4.652±3.346)ng/mL和0.055±0.037,ESCC组均较正常对照组显著升高(均为P<0.01)。ROC曲线分析显示,MMP1诊断ESCC的曲线下面积(AUC)为0.700(95%CI为0.624~0.776),当取最佳诊断截断值7.248 ng/mL时,特异度为83.3%,敏感度为52.7%。P53自身抗体诊断ESCC的AUC为0.713(95%CI为0.638~0.788),诊断截断值为0.080,特异度为83.3%,敏感度为49.5%。两者联合检测诊断ESCC的AUC为0.787(95%CI为0.720~0.854),特异度为83.3%,敏感度为66.7%,优于单独使用MMP1或P53自身抗体。在早期ESCC中,MMP1和P53自身抗体联合检测亦能获得较好的诊断效能,AUC为0.760,95%CI为0.663~0.857,特异度为83.3%,敏感度为66.7%。结论:ESCC患者血清MMP1和P53自身抗体水平均升高,二者联合检测有助于ESCC的早期诊断,可作为ESCC的诊断标志物。OBJECTIVE:To evaluate diagnostic values from the combined detection of serum MMP1 and P53 autoantibody in patients with esophageal squamous cell carcinoma(ESCC).METHODS:The study included 93 ESCC patients hospitalized in the Cancer hospital of Shantou University Medical College and 90 physical examination subjects as normal controls.Serum levels of MMP1 and P53 autoantibodies were measured by enzyme-linked immunosorbent assay(ELISA).The diagnostic value was evaluated using the receiver operating characteristic(ROC)curve.RESULTS:The serum levels of MMP1 and P53 autoantibodies of ESCC patients were higher than those in normal controls(P<0.01).ROC curves showed that the area under curve(AUC)of MMP1 in ESCC diagnosis was 0.700(95%CI:0.624-0.776).When the optimum diagnostic cutoff was 7.248 ng/mL,the sensitivity was 52.7%,and the specificity was 83.3%.Measurement of P53 autoantibodies demonstrated an AUC of 0.713(95%CI:0.638-0.788),with the diagnostic cutoff of 0.080 and a sensitivity/specificity of 49.5%/83.3%.The combination of MMP1 and P53 autoantibodies yielded an AUC of 0.787(95%CI:0.720-0.854),a sensitivity of 66.7%and a specificity of 83.3%.In early-stage ESCC,combined detection of MMP1 and P53 autoantibodies provided good diagnostic power(AUC=0.760;95%CI:0.663-0.857),with 66.7%sensitivity and 83.3%specificity.CONCLUSION:Serum MMP1 and P53 autoantibody were both over-expressed in ESCC patients.The use of both biomarkers was helpful in making early diagnosis of ESCC.

关 键 词：基质金属蛋白酶1 P53自身抗体 食管鳞状细胞癌 早期诊断 

分 类 号：R735.1[医药卫生—肿瘤]