一种适应夏季水产养殖水的硝化杆菌培养物分离及优化  

Isolation and Optimization of Culture Conditions of a Nitrobacter Enrichment Culture

作　　者：王殷娜 陈诚 蔡雨衡 夏茹婷 程凯 WANG Yinna;CHEN Cheng;CAI Yuheng;XIA Ruting;CHENG Kai(Hubei Key Laboratory of Ecological Restoration for River-Lakes and Algal Utilization,School of Civil Engineering,Architecture and Environment,Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学土木建筑与环境学院,河湖生态修复与藻类利用湖北省重点实验室,湖北武汉430068

出　　处：《环境科学与技术》2024年第4期44-53,共10页Environmental Science & Technology

基　　金：国家级大学生创新创业训练计划(202310500020);湖北工业大学教育发展基金会项目:养殖水体NOB增效技术研究(JJH2020069)。

摘　　要：为提高高温、高p H养殖水的亚硝态氮去除效果,该文从酿酒废水中筛选得到了只含有1种亚硝酸盐氧化菌(NOB)的富集培养物WY-8,通过16S rDNA序列和NxrA功能基因序列分析对富集培养物进行了鉴定,通过单因素试验优化了发酵参数,并在实际养殖水中测定了其亚硝态氮去除速率和倍增时间。结果表明,该NOB被鉴定为Nitrobacter winogradskyi,且其在富集培养物中的丰度高达96.27%。WY-8的最适发酵条件为:29~38℃、p H 7.0~9.0、盐度0.13%~0.63%、亚硝态氮浓度50~400 mg/L、硝态氮浓度<200 mg/L。在最适条件下,WY-8能在108 h内将138 mg/L的亚硝态氮转化为硝态氮,最大亚硝态氮去除速率高达4.38 mg/(L·h),最短倍增时间为7.5 h。WY-8在35℃、p H 8.5的实际养殖水中的最大亚硝态氮去除速率可达0.41 mg/(L·h),最短代时为7.8 h。研究表明,WY-8在夏季高pH养殖水体中具有良好的应用前景。In order to enhance the removal efficiency of nitrite in aquaculture water under high temperature and high pH con-ditions,an enrichment culture WY-8,containing only one type of nitrite-oxidizing bacteria(NOB),was screened from wine wastewater.The enrichment culture was identified through 16S rDNA sequence and NxrA functional gene sequence analysis.The fermentation parameters of the enrichment culture were optimized through single-factor experiments,and its nitrite removal ability and generation time in three actual aquaculture waters was measured.The results showed that the NOB was identified as Nitrobacter winogradskyi and its relative abundance was as high as 96.27%.The optimal fermentation parameters for WY-8 were 29~38℃,pH 7.0~9.0,salinity 0.13%~0.63%and 50~400 mg/L nitrite,nitrate<200 mg/L.Under the optimal conditions,the enrichment culture could convert 138 mg/L nitrite into nitrate in 108 h,with the maximum nitrite removal rate of 4.38 mg/(L·h),and the shortest generation time of 7.5 h.In aquaculture waters with a temperature of 35℃and a pH of 8.5,the maximum nitrite removal rate of the enrichment culture reached 0.41 mg/(L·h).The shortest generation time of the enrichment culture was 7.8 h in actual aquaculture waters.The above results indicated that the enrichment culture has a potential application prospects in aquaculture water with high pH and high water temperature in summer.

关 键 词：硝化细菌 亚硝态氮 高温 高pH 养殖水 生长 

分 类 号：X172[环境科学与工程—环境科学]