埃洛石纳米管对铜胁迫下小麦幼苗生长的影响  

Effect of Halloysite Nanotubes on Growth of Wheat under Copper Stress

作　　者：王敬营 王佳旭 陈丽娜[1] 王秀平[1] WANG Jingying;WANG Jiaxu;CHEN Lina;WANG Xiuping(Hebei Normal University of Science and Technology,Crop Adversities Biology Key Laboratory of Hebei Province,Qinhuangdao 066000,China)

机构地区：[1]河北科技师范学院河北省作物逆境生物学重点实验室,河北秦皇岛066000

出　　处：《河南农业科学》2024年第7期28-34,共7页Journal of Henan Agricultural Sciences

基　　金：河北省重点研发计划项目(21326507D)。

摘　　要：在水培条件下设置3个铜(Cu)处理[20 mg/L Cu^(2+)(20Cu)、50 mg/L Cu^(2+)(50Cu)和100 mg/L Cu^(2+)(100Cu)],研究外源添加50 mg/L埃洛石纳米管(HNTs)对Cu胁迫下小麦幼苗生长、气孔大小、叶绿体色素含量、根系氧化损伤、植株内Cu^(2+)含量的影响,以期为HNTs缓解重金属污染对作物生长的影响提供理论依据。结果表明,外源添加HNTs显著提高了Cu胁迫下小麦幼苗的株高、根长,20Cu+HNTs、50Cu+HNTs、100Cu+HNTs处理株高分别提高了13.10%、26.45%、28.30%,根长分别提高了62.87%、56.68%、92.34%;外源添加HNTs显著提高了Cu胁迫下小麦幼苗干、鲜质量,叶绿素、类胡萝卜素含量,根系活力,降低了根系氧化损伤程度,其中,叶绿素a含量分别提高了34.34%、18.09%、54.76%,叶绿素b含量分别提高了44.83%、25.93%、43.75%,根系活力分别提高了38.16%、339.48%、453.56%;与100Cu处理相比,100Cu+HNTs处理小麦幼苗根系、叶片中Cu^(2+)含量分别降低了19.18%、37.02%。综上,外源添加HNTs能够降低小麦幼苗中Cu^(2+)含量,缓解重金属Cu对小麦幼苗的毒性,促进其生长。Three copper(Cu)treatments[20 mg/L Cu^(2+)(20Cu),50 mg/L Cu^(2+)(50Cu)and 100 mg/L Cu^(2+)(100Cu)]were set up under hydroponics to study the effect of exogenous addition of 50 mg/L halloysite nanotubes(HNTs)on wheat seedling growth,stomata size,chloroplast pigment content,oxidative damage of roots and Cu^(2+)content in plants under Cu stress,so as to provide theoretical basis for HNTs to alleviate the effects of heavy metal pollution on crop growth.The results showed that exogenous addition of HNTs significantly increased the plant height and root length of wheat seedlings under Cu stress,the plant height of 20Cu+HNTs,50Cu+HNTs and 100Cu+HNTs treatments increased by 13.10%,26.45%and 28.30%,and the root length increased by 62.87%,56.68%and 92.34%,respectively.Exogenous addition of HNTs significantly increased the dry and fresh weight of wheat seedlings,chlorophyll and carotenoid contents of wheat seedlings under Cu stress,as well as root activity,and reduced the oxidative damage degree of roots,among which chlorophyll a content increased by 34.34%,18.09%and 54.76%,chlorophyll b content increased by 44.83%,25.93%and 43.75%,and root activity increased by 38.16%,339.48%and 453.56%,respectively.Compared with 100Cu treatment,the Cu^(2+)content in roots and leaves of wheat seedlings of 100Cu+HNTs treatment decreased by 19.18%and 37.02%,respectively.In conclusion,exogenous addition of HNTs can reduce Cu^(2+)content in wheat seedlings,alleviate the toxicity of heavy metal Cu to wheat seedlings,and promote their growth.

关 键 词：小麦 埃洛石纳米管 铜胁迫 生长发育 缓解 

分 类 号：S512[农业科学—作物学]