CDK1基因对肝癌细胞HepG2、Huh7增殖、迁移的影响及作用机制  

The effect and mechanism of CDK1 gene on proliferation and migration of hepatocellularcarcinoma HepG2 and Huh7 cells

作　　者：杨莹 张琪敏 郭钰梵 李慧鑫 张顺波 孙玉 YANG Ying;ZHANG Qimin;GUO Yufan;LI Huixin;ZHANG Shunbo;SUN Yu(Fenyang College of Shanxi Medical University,Shanxi Fenyang 032200,China)

机构地区：[1]山西医科大学汾阳学院,山西汾阳032200

出　　处：《现代肿瘤医学》2024年第16期2990-2998,共9页Journal of Modern Oncology

基　　金：山西省高等学校科技创新项目(编号:2022L673);山西医科大学汾阳学院校级项目(编号:2022C20)。

摘　　要：目的:基于生物信息学分析探讨CDK1表达与肝癌预后、临床特征的相关性,并通过实验进一步探讨其对肝癌细胞HepG2、Huh7增殖和迁移的影响。方法:通过检索TIMER数据库和GEPIA数据库,分析CDK1基因在肝癌和正常肝组织中的表达差异。借助UALCAN数据库验证CDK1蛋白在肝癌中的表达水平,运用R软件分析CDK1在GSE45267、GSE121247数据集中表达情况,采用单因素、多因素回归分析CDK1与肝癌临床病理参数的关系及预后价值,采用GEPIA和Kaplan-Meier Plotter数据库分析CDK1的表达与肝癌患者生存率的关系。将肝癌细胞HepG2、Huh7分为对照组和si_CDK1组(细胞转染CDK1-inhibitor),CCK-8法检测细胞增殖率,细胞划痕实验检测细胞愈合率,Transwell迁移实验分析计算细胞迁移数。通过LinkedOmics数据库探讨与CDK1共表达的正负相关基因,并进行通路富集分析。Western blot检测各组细胞中细胞周期、凋亡相关蛋白表达。结果:CDK1在肝癌组织中的表达显著高于正常肝组织,且CDK1为影响肝癌患者的独立危险因素,CDK1表达越高患者存活率和预后越差;CDK1共表达基因主要参与细胞周期、DNA复制等;与Control组相比,si_CDK1组肝癌细胞的增殖、愈合率和迁移能力明显降低,细胞周期蛋白CyclinD1、凋亡通路Bcl-2蛋白表达显著降低。结论:CDK1在肝癌组织中表达明显升高,并且与患者预后不良相关,沉默CDK1可以使肝癌细胞的增殖、迁移能力显著下降,引起细胞周期阻滞,促进细胞凋亡。Objective:To explore the correlation between CDK1 expression and prognosis and clinical features of hepatocellular carcinoma based on bioinformatics analysis,and further explored its effect on the proliferation and migration of hepatocellular carcinoma cells HepG2 and Huh7 by experiment.Methods:The differences in the expression of CDK1 gene in hepatocellular carcinoma and normal liver tissues were analyzed by searching TIMER database and GEPIA database.The expression level of CDK1 protein in hepatocellular carcinoma was verified with the help of UALCAN database,and the expression of CDK1 in GSE45267 and GSE121247 datasets was analyzed by using R software,and the relationship between CDK1 and clinicopathological parameters of hepatocellular carcinoma as well as the prognostic value were analyzed by using single-factor and multifactor regression,and the relationship between CDK1 expression and survival rate of hepatocellular carcinoma patients was analyzed by using GEPIA and Kaplan-Meier Plotter database.Hepatocellular carcinoma cells HepG2 and Huh7 were divided into control group and si_CDK1 group(cells transfected with CDK1-inhibitor),cell proliferation rate was detected by CCK-8 assay,cell healing rate was detected by cell scratch assay,and Transwell migration assay was analyzed to calculate the number of cell migration.Positive and negative genes co - expressed with CDK1 were explored by LinkedOmics database, and pathway enrichmentanalysis was performed. Western blot was used to detect cell cycle and apoptosis related protein expression ineach group of cells. Results: The expression of CDK1 in hepatocellular carcinoma was significantly higher than that innormal liver tissues,and CDK1 was an independent risk factor affecting hepatocellular carcinoma patients,and thehigher the expression of CDK1, the worse the survival and prognosis of the patients. CDK1 co - expressed genes weremainly involved in the cell cycle,DNA replication,etc. Compared with the Control group, the proliferation,healingrate, and migratory abi

关 键 词：CDK1 肝癌 增殖 迁移 

分 类 号：R735.7[医药卫生—肿瘤]