CST4和TGFB2在食管癌组织中的表达及临床意义  

The expression of CST4 and TGFB2 in esophageal cancer tissues and their clinical significance

作　　者：郭嘉仪 韩袁兴 宋正宇 张海平[1] 伊地力斯·阿吾提[1,3] GUO Jiayi;HAN Yuanxing;SONG Zhengyu;ZHANG Haiping;EDRISAWUT(Department of Thoracic Surgery,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830054,China;Imaging Center,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830054,China;Xinjiang Medical University Jointly Established the State Key Laboratory of Causes and Prevention of High Incidence in Central Asia,Xinjiang Urumqi 830054,China.)

机构地区：[1]新疆医科大学第一附属医院胸外科,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院影像中心,新疆乌鲁木齐830054 [3]新疆医科大学省部共建中亚高发病成因与防治国家重点实验室,新疆乌鲁木齐830054

出　　处：《现代肿瘤医学》2024年第14期2546-2554,共9页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:81960498);省部共建中亚高发病成因与防治国家重点实验室开放课题(编号:SKL-HIDCA-2021-21)。

摘　　要：目的:探讨半胱氨酸蛋白酶抑制剂S(cysteine protease inhibitor S,CST4)和转化生长因子β2(transforming growth factor β2,TGFB2)在食管癌组织中的表达及临床意义。方法:通过生物信息学分析,了解CST4和TGFB2在癌症中的作用。纳入2010-2019年于我院确诊为食管癌的患者68例,采用免疫组织化学的方法检测CST4和TGFB2的表达水平;用Kaplan-Meier和COX回归模型分析CST4和TGFB2对患者生存的影响。结果:通过生物信息学分析我们发现CST4和TGFB2基因主要与细胞黏附、迁移和凋亡有关。通过免疫组化实验我们发现CST4和TGFB2在食管癌组织中的阳性表达率显著高于正常食管组织。同时CST4和TGFB2与食管癌患者的T分期、N分期显著相关性。此外,我们对纳入的68例患者进行了5年的随访,中位生存时间为26个月。通过Kaplan-Meier生存分析我们发现在食管癌患者中CST4(中位生存时间=21个月,P=0.004)和TGFB2(中位生存时间=19个月,P=0.01)的阳性表达与较低的5年总体生存率(overall survival, OS)显著相关。并且当CST4和TGFB2共同表达阳性时患者5年OS最低(中位生存时间=16个月,P=0.004)。经COX回归模型分析发现CST4和TGFB2均为患者不良预后的独立危险因素。与此同时CST4和TGFB2的表达水平呈正相关。结论:CST4和TGFB2在食管癌组织中呈阳性表达,且两者的阳性表达可能与患者预后不良有关。因此,CST4和TGFB2可能是预测食管癌患者预后的潜在新标志物。Objective:To investigate the expression of cysteine protease inhibitor S(CST4)and transforming growth factorβ2(TGFB2)in esophageal cancer tissues and their clinical significance.Methods:Bioinformatics analysis was used to understand the role of CST4 and TGFB2 in cancer.The expression levels of CST4 and TGFB2 were detected by immunohistochemistry in 68 patients with esophageal cancer diagnosed in our hospital from 2010 to 2019.Kaplan-Meier and COX regression models were used to analyze the effects of CST4 and TGFB2 on patient survival.Results:We found that CST4 and TGFB2 genes were mainly related to cell adhesion,migration and apoptosis through biological information analysis.We found that the positive expression rates of CST4 and TGFB2 in esophageal cancer tissues were significantly higher than those in normal esophageal tissues by immunohistochemistry.At the same time,CST4 and TGFB2 were significantly correlated with T stage and N stage of esophageal cancer patients.In addition,we followed up 68 patients for 5 years,and the median survival time was 26 months.We found that positive expression of CST4(median survival time=21 months,P=0.004)and TGFB2(median survival time=19 months,P=0.01) was significantly associated with lower 5-year overall survival (OS) in patients with esophageal cancer by Kaplan-Meier survival analysis.And when CST4 and TGFB2 co-expressed positive,the 5-year OS of patients was the lowest (median survival time=16 months, P =0.004).We found that both CST4 and TGFB2 were independent risk factors for poor prognosis of patients analyzed by COX regression model.At the same time,the expression levels of CST4 and TGFB2 were positively correlated. Conclusion: CST4 and TGFB2 are positively expressed in esophageal cancer tissues,and their positive expression may be related to the poor prognosis of patients.Therefore,CST4 and TGFB2 may be potential new markers for predicting the prognosis of patients with esophageal cancer.

关 键 词：食管癌 CST4 TGFB2 预后 

分 类 号：R735.1[医药卫生—肿瘤]