PDCD4和miR-107在阴茎癌组织中的表达及与患者预后的关系  

The expression of PDCD4 and miR-107 in penile cancer tissue and their relationship with patient prognosis

作　　者：王志[1,2,3] 何长海 董彪 邱学德[3] 李保安[2] 赵琪 王鸿 朱海冬 刘俊峰 刘正道 吕明珠 江专新 WANG Zhi;HE Changhai;DONG Biao;QIU Xuede;LI Baoan;ZHAO Qi;WANG Hong;ZHU Haidong;LIU Junfeng;LIU Zhengdao;LYU Mingzhu;JIANG Zhuanxin(Department of Male,the Second Affiliated Hospital of Kunming Medical University,Yunnan Kunming 650000,China;Department of Urology,Nanyang First People's Hospital Affiliated to Henan University,Henan Nanyang 473000,China;Department of Urology,the Second Affiliated Hospital of Kunming Medical University,Yunnan Kunming 650000,China;Nanyang Medical College,Henan Nanyang 473000,China.)

机构地区：[1]昆明医科大学第二附属医院男性科,云南昆明650000 [2]河南大学附属南阳市第一人民医院泌尿外科,河南南阳473000 [3]昆明医科大学第二附属医院泌尿外科,云南昆明650000 [4]南阳医学高等专科学校,河南南阳473000

出　　处：《现代肿瘤医学》2024年第14期2620-2624,共5页Journal of Modern Oncology

摘　　要：目的:探究程序性细胞死亡因子4(PDCD4)及微小RNA-107(miR-107)在阴茎癌组织中的表达水平及与患者预后的关系。方法:选取2018年01月至2020年06月经本院确诊阴茎癌并行手术治疗患者32例,术中分别取患者阴茎癌组织(研究组)及癌旁正常阴茎组织(对照组)各32对,应用实时定量PCR(quantitative reverse transcription-PCR)检测两组PDCD4和miR-107的表达水平并分析其与临床病理参数及预后的关系,并采用Kaplan-Meier进行生存曲线分析及Logistic回归分析影响阴茎癌患者预后的因素。结果:研究组PDCD4表达量明显低于对照组,miR-107表达量明显高于对照组(P均<0.05);PDCD4和miR-107的表达与阴茎癌患者淋巴结转移、组织分级相关(P均<0.05),与年龄、吸烟史、饮酒史、肿瘤部位无关(P均>0.05);应用Kaplan-Meier法对生存率进行分析,miR-107低表达组生存率显著高于miR-107高表达组,PDCD4低表达组生存率低于PDCD4高表达组(P均<0.05)。多因素分析结果显示,TNM分期高、淋巴结转移、PDCD4低表达和miR-107高表达均是影响阴茎癌患者预后的独立危险因素(均P<0.05)。结论:阴茎癌患者miR-107呈异常高表达,PDCD4呈低表达,二者均是影响阴茎癌的独立危险因素且有望作为预测阴茎癌患者预后的重要指标。Objective:To explore the expression level of programmed cell death factor 4(PDCD4)and microRNA-107(miR-107)in penile cancer tissue and their relationship with patient prognosis.Methods:A research was made of 32 patients with penile cancer diagnosed in our hospital from January 2018 to June 2020,and 32 pairs of penile cancer tissue(study group)and normal penile tissue adjacent to cancer(control group)were taken during surgery.The expression levels of PDCD4 and miR-107 in the two groups were detected by real-time quantitative reverse transcription-PCR,and their relationship with clinicopathologic parameters and prognosis was analyzed.Kaplan-Meier survival curve analysis and Logistic regression analysis were used to analyze the factors affecting the prognosis of patients with penile cancer.Results:The expression of PDCD4 in the study group was significantly lower than that in the control group,and the expression of miR-107 was significantly higher than that in the control group(P<0.05).The expression level of PDCD4 and miR-107 was correlated with lymph node metastasis,histological grade of penis cancer(P<0.05).Kaplan-Meier method was used to analyze the survival rate.The survival rate of the low expression group of miR-107 was significantly higher than that of the high expression group of miR-107,and the survival rate of the low expression group of PDCD4 was lower than that of the high expression group of PDCD4( P <0.05).Multivariate analysis showed that high TNM stage,lymph node metastasis,low expression of PDCD4 and high expression of miR-107 were independent risk factors affecting the prognosis of patients with penile cancer (all P <0.05). Conclusion: The expression of miR-107 and PDCD4 in patients with penile cancer is abnormally high and low,which are expected to be used as important indicators to predict the prognosis of penile cancer.

关 键 词：程序性细胞死亡因子4 阴茎癌 微小RNA-107 预后 

分 类 号：R737.27[医药卫生—肿瘤]