应用植入式缓释泵实现大鼠脑室给药的操作方法及其改进  

Method and improvement of implantable osmotic pump for intraventricular drug administration in rats

作　　者：孙和勇 张刚利[1,2] 吴济良 曹培立 李硕 孙浩钦 SUN Heyong;ZHANG Gangli;WU Jiliang;CAO Peili;LI Shuo;SUN Haoqin(Department of Neurosurgery,the Fifth Clinical Medical College of Shanxi Medical University,Taiyuan 030012,China;Department of Neurosurgery,Shanxi Provincial People’s Hospital,Taiyuan 030012)

机构地区：[1]山西医科大学第五临床医学院神经外科,太原030012 [2]山西省人民医院神经外科,太原030012

出　　处：《中国比较医学杂志》2024年第7期89-94,共6页Chinese Journal of Comparative Medicine

基　　金：山西省自然科学基金(202203021211060)。

摘　　要：目的介绍应用植入式缓释泵实现脑室给药的实验技术并予以改进。方法选择8周龄雄性SD大鼠,准备所需器材及试剂。首先将缓释泵组装并孵育至工作状态,然后进行植入手术操作。实验动物麻醉后备皮,手术暴露颅骨上表面,使用脑立体定位仪定位脑室正上方一点,在该点用高速颅钻钻1个小孔。先将泵体埋置于颈部皮下,然后将针头插入小孔中,并用牙科水泥固定,凝固后剪去针头基座,逐层缝合皮下软组织和头皮,将动物放回笼内单独饲养。结果缓释泵泵体成功植入大鼠颈部皮下,针头牢固固定于颅骨,导管接口未断开。取出完整脑组织检查,可见穿刺点及针道周围无明显血肿,脑室内及周围组织可见蓝色染料,表明本方法能够成功将药物送达脑室。结论通过引入脑立体定位仪辅助定位,并对此技术的操作流程进行改进,使植入操作更加精准、安全,具有较高的脑室给药成功率。Objective To introduce and enhance an experimental technique for intraventricular drug delivery via an implantable osmotic pump.Methods Eight⁃week⁃old male SD rats were selected and the requisite equipment and reagents were prepared.The osmotic pump was assembled and brought to operational status before conducting the implantation surgery.Following anesthesia,the rats underwent skin preparation and the upper surface of the skull was surgically exposed.A point directly above the ventricle was located using a brain stereotaxic apparatus,and a small hole was drilled at that location with a high⁃speed cranial drill.The pump body was then implanted subcutaneously in the neck and the needle was inserted into the drilled hole,and secured with dental cement.Once solidified,the needle base was removed,the subcutaneous soft tissue and scalp were sutured in layers,and the animal was returned to its cage for rearing in isolation.Results The osmotic pump was successfully implanted subcutaneously in the rat’s neck,the needle was securely fixed to the skull,and the catheter interface remained intact.The rats were sacrificed and the brain tissue was removed.Examination of the extracted brain tissue revealed no significant hematoma around the puncture site or needle tract,and the presence of blue dye in the ventricular and adjacent tissues indicated successful drug delivery to the ventricle.Conclusions The introduction of a brain stereotaxic apparatus to aid localization,coupled with enhancements to the operational procedure,may improve the accuracy and safety of the implantation process resulting in a high success rate for intraventricular drug administration.

关 键 词：植入式缓释泵 脑室给药 脑立体定位仪 实验方法 技术改进 

分 类 号：R-33[医药卫生]