动物细胞永生化研究进展  

Research Progress in the Immortalization of Animal Cells

作　　者：张震宇 靳莉武 王婧尊 田玲 乔自林[1,2,4] 杨迪[1,2,5] 阿依木古丽·阿不都热依木 ZHANG Zhen-yu;JIN Li-wu;WANG Jing-zun;TIAN Ling;QIAO Zi-in;YANG Di;AYIMUGULI Abudureyimu(Gansu Tech Innovation Center of Animal Cell,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030;Engineering Research Center for Key Technologies and Industrialization of Cell-Based Vaccines,Ministry of Education,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030;College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030;Key Laboratory of Bioengineering and Technology State Ethnic Affairs Commission,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030;Department of Experiment Teaching,Northwest Minzu University,Lanzhou 730030)

机构地区：[1]西北民族大学生物医学研究中心甘肃省动物细胞技术创新中心,兰州730030 [2]西北民族大学生物医学研究中心细胞基质疫苗关键技术与产业化教育部工程研究中心,兰州730030 [3]西北民族大学生命科学与工程学院,兰州730030 [4]西北民族大学生物医学研究中心生物工程与技术国家民委重点实验室,兰州730030 [5]西北民族大学实验教学部,兰州730030

出　　处：《生物技术通报》2024年第7期78-89,共12页Biotechnology Bulletin

基　　金：甘肃省科技计划重点研发项目(23YFFA0071);中央高校基本科研业务费项目(31920220070,31920230001);甘肃省科技计划项目(22JR11RA239)。

摘　　要：细胞永生化是指通过各种手段、方式来突破原代细胞衰老的限制,延长细胞寿命,实现细胞无限复制增殖,是当前生物医学领域研究的一个热点与难点。永生化细胞株/系可用于生物制品研发、衰老机制探索以延伸生命等;然而目前永生化细胞种类不多、永生化技术相对较难,肿瘤风险以及动物福利伦理等原因,还不能满足研发需求。目的细胞永生化不仅可以永久保存动物细胞遗传资源,还对多种疾病的诊断、治疗及细胞基质疫苗的研发等提供支持。本文就动物细胞永生化的原理、永生化机制及方法的研究最新进展进行综述,为未来的生物医学研究和应用提供参考。Cell immortalization refers to breaking through the limitations of primary cell aging,extending cell lifespan,and achieving unlimited cell replication and proliferation through various means and methods,which is a hot and difficult research topic in the current biomedical field.Immortalized cell lines can be used for the development of biological products,exploration of aging mechanisms to extend life,etc.However,due to the limited variety of immortalized cells,relatively difficult immortalization technology,tumor risk,and animal welfare ethics,it still cannot meet the research and developing needs.The purpose of immortalizing cells is not only to permanently preserve the genetic resources of animal cells,but also to provide support for the diagnosis and treatment of various diseases,as well as to develop cell matrix for vaccines.This article reviews the latest progresses in the principles,mechanisms,and methods of immortaling animal cells,providing reference for future biomedical research and applications.

关 键 词：动物细胞 细胞永生化 永生化机制 永生化方法 

分 类 号：Q813[生物学—生物工程]