绵羊肺炎支原体GH3-3株全基因组测序及生物信息学分析  

Whole Genome Sequencing and Bioinformatics Analysis of Mycoplasma ovipneumoniae GH3-3 Strain

作　　者：田彤彤 葛家振 高鹏程[1] 李学瑞[1] 宋国栋 郑福英[1] 储岳峰[1,2] TIAN Tong-tong;GE Jia-zhen;GAO Peng-cheng;LI Xue-rui;SONG Guo-dong;ZHENG Fu-ying;CHU Yue-feng(State Key Laboratory for Animal Disease Control and Prevention,College of Veterinary Medicine,Lanzhou University,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730000;College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052)

机构地区：[1]中国农业科学院兰州兽医研究所兰州大学动物医学与生物安全学院动物疫病防控全国重点实验室,兰州730000 [2]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《生物技术通报》2024年第7期323-334,共12页Biotechnology Bulletin

基　　金：国家重点研发计划(2022YFD1800704);中国农业科学院兰州兽医研究所“元亨人才”计划项目(所级青年英才培育项目)(NKLS2020-119);甘肃省科技重大专项课题(23ZDNA007)。

摘　　要：【目的】全面了解绵羊肺炎支原体GH3-3株的基因序列,研究其潜在的致病机制及其复制、转录、翻译过程的调控机制。【方法】采用体外培养,利用细菌基因组DNA提取试剂盒提取绵羊肺炎支原体GH3-3株基因组DNA,进行全基因组测序。【结果】绵羊肺炎支原体GH3-3株基因组大小为1060772 bp,GC含量为29.66%,基因组组分分析后发现,GH3-3株的基因组含有730个编码基因,总长度为914379 bp,平均长度为1252.57 bp,占基因组全长的86.2%。串联重复序列共149个,总长为20926 bp,占基因组全长的1.97%。微卫星DNA序列102个,tRNA 30个,rRNA 3个。在NR、SwissProt、GOG、KEGG、GO、CARD、CAZy、PHI、TCDB、RMS数据库中,分别有719、459、473、394、449、33、5、180、113、59个基因被注释;在VFDB数据库中,共注释到了76个毒力因子相关的基因。将基因组序列提交至NCBI网站,获得登录号为:PRJNA1051969。【结论】获得了绵羊肺炎支原体GH3-3株完整的基因组信息,预测和注释了其基因的功能,明确了GH3-3株以及与国内外其他绵羊肺炎支原体菌株之间的遗传进化关系。【Objective】This study is aimed to decode the genomic sequence of Mycoplasma ovipneumoniae strain GH3-3,to investigate its pathogenic potential and to elucidate the regulatory mechanisms of its replication,transcription,and translation.【Method】M.ovipneumoniae GH3-3 strain was cultured in vitro.When it reached the late logarithmic stage of growth,the genomic DNA of M.ovipneumoniae GH3-3 strain was extracted with bacterial genomic DNA extraction kit and sent for whole genome sequencing and commentary.【Result】The genome size of GH3-3 strains of M.ovipneumoniae was 1060772 bp,and the GC content was 29.66%.The genome component analysis showed that the genome of GH3-3 strain contained 730 coding genes,with a total length of 914379 bp and an average length of 1252.57 bp,accounting for 86.2%of the total genome length.There were 149 tandem repeats with a total length of 20926 bp,accounting for 1.97%of the total length of the genome.There were 102 microsatellite DNA sequences,30 tRNA sequences,and 3 rRNA sequences.The 719,459,473,394,449,33,5,180,113 and 59 genes were annotated in NR,SwissProt,GOG,KEGG,GO,CARD,CAZy,PHI,TCDB,and RMS databases,respectively.A total of 76 virulence factor-related genes were annotated.The genome sequence was submitted to the NCBI website with the login number:PRJNA1051969.【Conclusion】The complete genome information of M.ovipneumoniae GH3-3 strain was acquired,and the gene function was predicted and annotated.Furthermore,the genetic and evolutionary relationships between GH3-3 strain and other strains of M.ovipneumoniae were elucidated globally.

关 键 词：绵羊肺炎支原体 GH3-3株 全基因组测序 毒力因子 耐药基因 

分 类 号：S858.26[农业科学—临床兽医学]